1ngx
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1ngx.gif|left|200px]] | [[Image:1ngx.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1ngx", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_1ngx| PDB=1ngx | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Chimeric Germline Fab 7g12 with jeffamine fragment bound''' | '''Chimeric Germline Fab 7g12 with jeffamine fragment bound''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGX OCA]. | |
==Reference== | ==Reference== | ||
Structural evidence for substrate strain in antibody catalysis., Yin J, Andryski SE, Beuscher AE 4th, Stevens RC, Schultz PG, Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):856-61. Epub 2003 Jan 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12552112 12552112] | Structural evidence for substrate strain in antibody catalysis., Yin J, Andryski SE, Beuscher AE 4th, Stevens RC, Schultz PG, Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):856-61. Epub 2003 Jan 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12552112 12552112] | ||
| - | [[Category: ]] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Andryski, S E.]] | [[Category: Andryski, S E.]] | ||
[[Category: Beuscher, A B.]] | [[Category: Beuscher, A B.]] | ||
| Line 30: | Line 25: | ||
[[Category: Stevens, R C.]] | [[Category: Stevens, R C.]] | ||
[[Category: Yin, J.]] | [[Category: Yin, J.]] | ||
| - | [[Category: | + | [[Category: Antibody]] |
| - | [[Category: | + | [[Category: Immunoglobulin]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:31:06 2008'' | |
| - | + | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 23:31, 2 May 2008
Chimeric Germline Fab 7g12 with jeffamine fragment bound
Overview
The crystal structure of the Michaelis complex between the Fab fragment of ferrochelatase antibody 7G12 and its substrate mesoporphyrin has been solved to 2.6-A resolution. The antibody-bound mesoporphyrin clearly adopts a nonplanar conformation and reveals that the antibody catalyzes the porphyrin metallation reaction by straining/distorting the bound substrate toward the transition-state configuration. The crystal structures of the Fab fragment of the germ-line precursor antibody to 7G12 and its complex with the hapten N-methylmesoporphyrin have also been solved. A comparison of these structures with the corresponding structures of the affinity-matured antibody 7G12 reveals the molecular mechanism by which the immune system evolves binding energy to catalyze this reaction.
About this Structure
Full crystallographic information is available from OCA.
Reference
Structural evidence for substrate strain in antibody catalysis., Yin J, Andryski SE, Beuscher AE 4th, Stevens RC, Schultz PG, Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):856-61. Epub 2003 Jan 24. PMID:12552112 Page seeded by OCA on Sat May 3 02:31:06 2008
