This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

2xsa

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 09:04, 10 October 2018

OgOGA apostructure

Structural highlights

2xsa is a 1 chain structure with sequence from Ocegh. The September 2011 RCSB PDB Molecule of the Month feature on O-GlcNAc Transferase by David Goodsell is 10.2210/rcsb_pdb/mom_2011_9. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	2xsb
Activity:	Beta-N-acetylhexosaminidase, with EC number 3.2.1.52
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[Q2CEE3_OCEGH] Cleaves GlcNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrate (in vitro).^[1]

Publication Abstract from PubMed

Modification of cellular proteins with O-GlcNAc (O-linked N-acetylglucosamine) competes with protein phosphorylation and regulates a plethora of cellular processes. O-GlcNAcylation is orchestrated by two opposing enzymes, O-GlcNAc transferase and OGA (O-GlcNAcase or beta-N-acetylglucosaminidase), which recognize their target proteins via as yet unidentified mechanisms. In the present study, we uncovered the first insights into the mechanism of substrate recognition by human OGA. The structure of a novel bacterial OGA orthologue reveals a putative substrate-binding groove, conserved in metazoan OGAs. Guided by this structure, conserved amino acids lining this groove in human OGA were mutated and the activity on three different substrate proteins [TAB1 (transforming growth factor-beta-activated protein kinase 1-binding protein 1), FoxO1 (forkhead box O1) and CREB (cAMP-response-element-binding protein)] was tested in an in vitro deglycosylation assay. The results provide the first evidence that human OGA may possess a substrate-recognition mechanism that involves interactions with O-GlcNAcylated proteins beyond the GlcNAc-binding site, with possible implications for differential regulation of cycling of O-GlcNAc on different proteins.

Human OGA binds substrates in a conserved peptide recognition groove.,Schimpl M, Schuttelkopf AW, Borodkin VS, van Aalten DM Biochem J. 2010 Oct 25;432(1):1-7. PMID:20863279^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Schimpl M, Schuttelkopf AW, Borodkin VS, van Aalten DM. Human OGA binds substrates in a conserved peptide recognition groove. Biochem J. 2010 Oct 25;432(1):1-7. PMID:20863279 doi:10.1042/BJ20101338
↑ Schimpl M, Schuttelkopf AW, Borodkin VS, van Aalten DM. Human OGA binds substrates in a conserved peptide recognition groove. Biochem J. 2010 Oct 25;432(1):1-7. PMID:20863279 doi:10.1042/BJ20101338

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2xsa"

@@ Line 1: / Line 1: @@
-==OGOGA APOSTRUCTURE==
+==OgOGA apostructure==
 <StructureSection load='2xsa' size='340' side='right' caption='[[2xsa]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2xsa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Oceanicola_granulosus_htcc2516 Oceanicola granulosus htcc2516]. The September 2011 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''O-GlcNAc Transferase''  by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2011_9 10.2210/rcsb_pdb/mom_2011_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XSA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XSA FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2xsa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ocegh Ocegh]. The September 2011 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''O-GlcNAc Transferase''  by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2011_9 10.2210/rcsb_pdb/mom_2011_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XSA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XSA FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2xsb|2xsb]]</td></tr>
@@ Line 26: / Line 26: @@
 [[Category: Beta-N-acetylhexosaminidase]]
 [[Category: O-GlcNAc Transferase]]
-[[Category: Oceanicola granulosus htcc2516]]
+[[Category: Ocegh]]
 [[Category: RCSB PDB Molecule of the Month]]
-[[Category: Aalten, D M.F Van]]
+[[Category: Aalten, D M.F van]]
 [[Category: Schuttelkopf, A W]]
 [[Category: Glycosyl hydrolase]]

2xsa

From Proteopedia

Revision as of 09:04, 10 October 2018

OgOGA apostructure

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox