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| | ==Crystal Structure of Pseudomonas aeruginosa transcriptional regulator PA2196 bound to its operator DNA== | | ==Crystal Structure of Pseudomonas aeruginosa transcriptional regulator PA2196 bound to its operator DNA== |
| - | <StructureSection load='4l62' size='340' side='right' caption='[[4l62]], [[Resolution|resolution]] 2.90Å' scene=''> | + | <StructureSection load='4l62' size='340' side='right'caption='[[4l62]], [[Resolution|resolution]] 2.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4l62]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L62 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4L62 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4l62]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L62 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4L62 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PA2196 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4l62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l62 OCA], [https://pdbe.org/4l62 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4l62 RCSB], [https://www.ebi.ac.uk/pdbsum/4l62 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4l62 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4l62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l62 OCA], [http://pdbe.org/4l62 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4l62 RCSB], [http://www.ebi.ac.uk/pdbsum/4l62 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4l62 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9I1S1_PSEAE Q9I1S1_PSEAE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 4l62" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 4l62" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Tetracycline repressor protein 3D structures|Tetracycline repressor protein 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Pseae]] | + | [[Category: Large Structures]] |
| - | [[Category: Choe, J W]] | + | [[Category: Pseudomonas aeruginosa PAO1]] |
| - | [[Category: Kim, Y W]] | + | [[Category: Choe JW]] |
| - | [[Category: Dna binding]] | + | [[Category: Kim YW]] |
| - | [[Category: Transcription-dna complex]]
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| Structural highlights
Function
Q9I1S1_PSEAE
Publication Abstract from PubMed
Pseudomonas aeruginosa is a major opportunistic human pathogen. PA2196 from P. aeruginosa is a member of TetR family of transcriptional repressors, which is involved in adaptation to environmental changes as well as bacterial antibiotic resistance. PA2196 consists of nine alpha-helical bundles divided into two separate domains. The N-terminal domain, called the DNA-binding domain, is composed of helices alpha1-alpha3 and has a helix-turn-helix motif. The C-terminal domain, called the ligand-binding domain, has a hydrophobic pocket for ligand binding. Here, PA2196 was shown to bind to a 25bp semi-palindromic dsDNA located in the upstream region of its own gene. The crystal structure of the PA2196-25mer dsDNA complex determined at a resolution of 2.9A revealed that two dimers of PA2196 bound to one dsDNA, with each monomer interacting with the major groove of DNA. Especially, residues in helix alpha3, including Lys41, Gly42, Ser43, and Tyr45, interacted mainly with the base and phosphate backbone of dsDNA. PA2196 underwent large conformational changes upon DNA binding, as the distances between DNA-binding domains measured between two G42s in subunits A and B decreased from 41.7A to 36.8A. Our crystal structure of PA2196-25mer dsDNA complex revealed that PA2196 is similar to QacR in that two dimers bound to one dsDNA through specific interactions.
Crystal structure of Pseudomonas aeruginosa transcriptional regulator PA2196 bound to its operator DNA.,Kim Y, Kang Y, Choe J Biochem Biophys Res Commun. 2013 Oct 18;440(2):317-21. doi:, 10.1016/j.bbrc.2013.09.074. Epub 2013 Sep 23. PMID:24070609[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Kim Y, Kang Y, Choe J. Crystal structure of Pseudomonas aeruginosa transcriptional regulator PA2196 bound to its operator DNA. Biochem Biophys Res Commun. 2013 Oct 18;440(2):317-21. doi:, 10.1016/j.bbrc.2013.09.074. Epub 2013 Sep 23. PMID:24070609 doi:http://dx.doi.org/10.1016/j.bbrc.2013.09.074
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