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| | ==Atomic Resolution Structure of PfGrx1== | | ==Atomic Resolution Structure of PfGrx1== |
| - | <StructureSection load='4mzc' size='340' side='right' caption='[[4mzc]], [[Resolution|resolution]] 0.95Å' scene=''> | + | <StructureSection load='4mzc' size='340' side='right'caption='[[4mzc]], [[Resolution|resolution]] 0.95Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4mzc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Plaf7 Plaf7]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4kjf 4kjf]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MZC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MZC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4mzc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4kjf 4kjf]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MZC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MZC FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MPO:3[N-MORPHOLINO]PROPANE+SULFONIC+ACID'>MPO</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MPO:3[N-MORPHOLINO]PROPANE+SULFONIC+ACID'>MPO</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4hjm|4hjm]], [[3c1r|3c1r]], [[3ctg|3ctg]], [[1kte|1kte]], [[4mzb|4mzb]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mzc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mzc OCA], [https://pdbe.org/4mzc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mzc RCSB], [https://www.ebi.ac.uk/pdbsum/4mzc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mzc ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GRX1, PFC0271c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36329 PLAF7])</td></tr>
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| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arsenate_reductase_(glutaredoxin) Arsenate reductase (glutaredoxin)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.20.4.1 1.20.4.1] </span></td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mzc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mzc OCA], [http://pdbe.org/4mzc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mzc RCSB], [http://www.ebi.ac.uk/pdbsum/4mzc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mzc ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9NLB2_PLAF7 Q9NLB2_PLAF7] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Plaf7]] | + | [[Category: Large Structures]] |
| - | [[Category: Sharma, A]] | + | [[Category: Plasmodium falciparum 3D7]] |
| - | [[Category: Yogavel, M]] | + | [[Category: Sharma A]] |
| - | [[Category: Active site]] | + | [[Category: Yogavel M]] |
| - | [[Category: Glutathione]]
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| - | [[Category: Oxidoreductase]]
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| - | [[Category: Plasticity]]
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| - | [[Category: Redox enzyme]]
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| - | [[Category: Sulfur-sad]]
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| - | [[Category: Trx fold]]
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| Structural highlights
Function
Q9NLB2_PLAF7
Publication Abstract from PubMed
Glutaredoxins (Grxs) are redox proteins that use glutathione ((gamma)Glu-Cys-Gly; GSH) as a cofactor. Plasmodium falciparum has one classic dithiol (CXXC) glutaredoxin (glutaredoxin 1; PfGrx1) and three monothiol (CXXS) Grx-like proteins (GLPs), which have five residue insertions prior to the active-site Cys. Here, the crystal structure of PfGrx1 has been determined by the sulfur single-wavelength anomalous diffraction (S-SAD) method utilizing intrinsic protein and solvent S atoms. Several residues were modelled with alternate conformations, and an alternate position was refined for the active-site Cys29 owing to radiation damage. The GSH-binding site is occupied by water polygons and buffer molecules. Structural comparison of PfGrx1 with other Grxs and Grx-like proteins revealed that the GSH-binding motifs (CXXC/CXXS, TVP, CDD, Lys26 and Gln/Arg63) are structurally conserved. Both the monothiol and dithiol Grxs possess three conserved water molecules; two of these were located in the GSH-binding site. PfGrx1 has several polar and charged amino-acid substitutions that provide structurally important additional hydrogen bonds and salt bridges missing in other Grxs.
Atomic resolution crystal structure of glutaredoxin 1 from Plasmodium falciparum and comparison with other glutaredoxins.,Yogavel M, Tripathi T, Gupta A, Banday MM, Rahlfs S, Becker K, Belrhali H, Sharma A Acta Crystallogr D Biol Crystallogr. 2014 Jan;70(Pt 1):91-100. doi:, 10.1107/S1399004713025285. Epub 2013 Dec 24. PMID:24419382[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Yogavel M, Tripathi T, Gupta A, Banday MM, Rahlfs S, Becker K, Belrhali H, Sharma A. Atomic resolution crystal structure of glutaredoxin 1 from Plasmodium falciparum and comparison with other glutaredoxins. Acta Crystallogr D Biol Crystallogr. 2014 Jan;70(Pt 1):91-100. doi:, 10.1107/S1399004713025285. Epub 2013 Dec 24. PMID:24419382 doi:http://dx.doi.org/10.1107/S1399004713025285
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