1nog
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1nog.gif|left|200px]] | [[Image:1nog.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1nog", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_1nog| PDB=1nog | SCENE= }} | |
| - | | | + | |
| - | | | + | |
| - | }} | + | |
'''Crystal Structure of Conserved Protein 0546 from Thermoplasma Acidophilum''' | '''Crystal Structure of Conserved Protein 0546 from Thermoplasma Acidophilum''' | ||
| Line 36: | Line 33: | ||
[[Category: Saridakis, V.]] | [[Category: Saridakis, V.]] | ||
[[Category: Xu, X.]] | [[Category: Xu, X.]] | ||
| - | [[Category: | + | [[Category: Mcsg]] |
| - | [[Category: | + | [[Category: Midwest center for structural genomic]] |
| - | [[Category: | + | [[Category: Protein structure initiative]] |
| - | [[Category: | + | [[Category: Psi]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:46:50 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 23:46, 2 May 2008
Crystal Structure of Conserved Protein 0546 from Thermoplasma Acidophilum
Overview
ATP:cobalamin adenosyltransferase MMAB was recently identified as the gene responsible for a disorder of cobalamin metabolism in humans (cblB complementation group). The crystal structure of the MMAB sequence homologue from Thermoplasma acidophilum (TA1434; GenBank identification number gi|16082403) was determined to a resolution of 1.5 A. TA1434 was confirmed to be an ATP:cobalamin adenosyltransferase, which depended absolutely on divalent metal ions (Mg2+ > Mn2+ > Co2+) and only used ATP or dATP as adenosyl donors. The apparent Km of TA1434 was 110 microM (kcat = 0.23 s(-1)) for ATP, 140 microM (kcat = 0.11 s(-1)) for dATP, and 3 microM (kcat = 0.18 s(-1)) for cobalamin. TA1434 is a trimer in solution and in the crystal structure, with each subunit composed of a five-helix bundle. The location of disease-related point mutations and other residues conserved among the homologues of TA1434 suggest that the active site lies at the junctions between the subunits. Mutations in TA1434 that correspond to the disease-related mutations resulted in proteins that were inactive for ATP:cobalamin adenosyltransferase activity in vitro, confirming that these mutations define the molecular basis of the human disease.
About this Structure
1NOG is a Single protein structure of sequence from Thermoplasma acidophilum. Full crystallographic information is available from OCA.
Reference
The structural basis for methylmalonic aciduria. The crystal structure of archaeal ATP:cobalamin adenosyltransferase., Saridakis V, Yakunin A, Xu X, Anandakumar P, Pennycooke M, Gu J, Cheung F, Lew JM, Sanishvili R, Joachimiak A, Arrowsmith CH, Christendat D, Edwards AM, J Biol Chem. 2004 May 28;279(22):23646-53. Epub 2004 Mar 25. PMID:15044458 Page seeded by OCA on Sat May 3 02:46:50 2008
Categories: Single protein | Thermoplasma acidophilum | Arrowsmith, C H. | Christendat, D. | Edwards, A M. | Gu, J. | Iakounine, A. | Joachimiak, A. | MCSG, Midwest Center for Structural Genomics. | Pennycooke, M. | Sanishvili, R. | Saridakis, V. | Xu, X. | Mcsg | Midwest center for structural genomic | Protein structure initiative | Psi | Structural genomic
