4r9t

From Proteopedia

(Difference between revisions)

Revision as of 10:30, 1 May 2019

L-ficolin complexed to sulphates

Structural highlights

4r9t is a 3 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , ,
Related:	2j0g, 2j0h, 2j0y, 2j3o, 4r9j
Gene:	FCN2, FCNL (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[FCN2_HUMAN] May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc-binding lectin. Enhances phagocytosis of S.typhimurium by neutrophils, suggesting an opsonic effect via the collagen region.^[1]

Publication Abstract from PubMed

Ficolin-2 has been reported to bind to DNA and heparin, but the mechanism involved has not been thoroughly investigated. X-ray studies of the ficolin-2 fibrinogen-like domain in complex with several new ligands now show that sulfate and phosphate groups are prone to bind to the S3 binding site of the protein. Composed of Arg132, Asp133, Thr136 and Lys221, the S3 site was previously shown to mainly bind N-acetyl groups. Furthermore, DNA and heparin compete for binding to ficolin-2. Mutagenesis studies reveal that Arg132, and to a lesser extent Asp133, are important for this binding property. The versatility of the S3 site in binding N-acetyl, sulfate and phosphate groups is discussed through comparisons with homologous fibrinogen-like recognition proteins.

Human ficolin-2 recognition versatility extended: An update on the binding of ficolin-2 to sulfated/phosphated carbohydrates.,Laffly E, Lacroix M, Martin L, Vassal-Stermann E, Thielens NM, Gaboriaud C FEBS Lett. 2014 Dec 20;588(24):4694-700. doi: 10.1016/j.febslet.2014.10.042. Epub, 2014 Nov 11. PMID:25447524^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Matsushita M, Endo Y, Fujita T. Cutting edge: complement-activating complex of ficolin and mannose-binding lectin-associated serine protease. J Immunol. 2000 Mar 1;164(5):2281-4. PMID:10679061
↑ Laffly E, Lacroix M, Martin L, Vassal-Stermann E, Thielens NM, Gaboriaud C. Human ficolin-2 recognition versatility extended: An update on the binding of ficolin-2 to sulfated/phosphated carbohydrates. FEBS Lett. 2014 Dec 20;588(24):4694-700. doi: 10.1016/j.febslet.2014.10.042. Epub, 2014 Nov 11. PMID:25447524 doi:http://dx.doi.org/10.1016/j.febslet.2014.10.042

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4r9t"

@@ Line 1: / Line 1: @@
 ==L-ficolin complexed to sulphates==
-<StructureSection load='4r9t' size='340' side='right' caption='[[4r9t]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
+<StructureSection load='4r9t' size='340' side='right'caption='[[4r9t]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4r9t]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R9T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R9T FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4r9t]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R9T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R9T FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2j0g|2j0g]], [[2j0h|2j0h]], [[2j0y|2j0y]], [[2j3o|2j3o]], [[4r9j|4r9j]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FCN2, FCNL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r9t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r9t OCA], [http://pdbe.org/4r9t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4r9t RCSB], [http://www.ebi.ac.uk/pdbsum/4r9t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4r9t ProSAT]</span></td></tr>
 </table>
@@ Line 26: / Line 27: @@
 __TOC__
 </StructureSection>
+[[Category: Human]]
+[[Category: Large Structures]]
 [[Category: Gaboriaud, C]]
 [[Category: Lacroix, M]]

4r9t

From Proteopedia

Revision as of 10:30, 1 May 2019

L-ficolin complexed to sulphates

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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