4kfv

From Proteopedia

(Difference between revisions)

Revision as of 11:56, 30 November 2022

Structural insight into Golgi membrane stacking by GRASP65 and GRASP55

Structural highlights

4kfv is a 1 chain structure with sequence from Rattus norvegicus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GORS1_RAT Key structural protein of the Golgi apparatus (By similarity). The membrane cisternae of the Golgi apparatus adhere to each other to form stacks, which are aligned side by side to form the Golgi ribbon (By similarity). Acting in concert with GORASP2/GRASP55, is required for the formation and maintenance of the Golgi ribbon, and may be dispensable for the formation of stacks (By similarity). However, other studies suggest that GORASP1 plays an important role in assembly and membrane stacking of the cisternae, and in the reassembly of Golgi stacks after breakdown during mitosis (PubMed:12839990, PubMed:23940043, PubMed:9346242). Caspase-mediated cleavage of GORASP1 is required for fragmentation of the Golgi during apoptosis (PubMed:11815631, PubMed:12015985). Also mediates, via its interaction with GOLGA2/GM130, the docking of transport vesicles with the Golgi membranes (By similarity). Mediates ER stress-induced unconventional (ER/Golgi-independent) trafficking of core-glycosylated CFTR to cell membrane (By similarity).[UniProtKB:Q91X51][UniProtKB:Q9BQQ3]^[1] ^[2] ^[3] ^[4] ^[5]

Publication Abstract from PubMed

The stacking of Golgi cisternae involves GRASP65 and GRASP55. The oligomerization of the N-terminal GRASP domain of these proteins, which consists of two tandem PDZ domains, is required to tether the Golgi membranes. However, the molecular basis for GRASP assembly is unclear. Here, we determined the crystal structures of the GRASP domain of GRASP65 and GRASP55. The structures reveal similar homotypic interactions: the GRASP domain forms a dimer in which the peptide-binding pockets of the two neighboring PDZ2 domains face each other, and the dimers are further connected by the C-terminal tail of one GRASP domain inserting into the binding pocket of the PDZ1 domain in another dimer. Biochemical analysis suggests that both types of contacts are relatively weak but are needed in combination for GRASP-mediated Golgi stacking. Our results unveil a novel mode of membrane tethering by GRASP proteins and provide insight into the mechanism of Golgi stacking.

Structural insight into Golgi membrane stacking by GRASP65 and GRASP55 proteins.,Feng Y, Yu W, Li X, Lin S, Zhou Y, Hu J, Liu X J Biol Chem. 2013 Sep 27;288(39):28418-27. doi: 10.1074/jbc.M113.478024. Epub, 2013 Aug 12. PMID:23940043^[6]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lane JD, Lucocq J, Pryde J, Barr FA, Woodman PG, Allan VJ, Lowe M. Caspase-mediated cleavage of the stacking protein GRASP65 is required for Golgi fragmentation during apoptosis. J Cell Biol. 2002 Feb 4;156(3):495-509. Epub 2002 Jan 28. PMID:11815631 doi:http://dx.doi.org/10.1083/jcb.200110007
↑ Sutterlin C, Hsu P, Mallabiabarrena A, Malhotra V. Fragmentation and dispersal of the pericentriolar Golgi complex is required for entry into mitosis in mammalian cells. Cell. 2002 May 3;109(3):359-69. doi: 10.1016/s0092-8674(02)00720-1. PMID:12015985 doi:http://dx.doi.org/10.1016/s0092-8674(02)00720-1
↑ Wang Y, Seemann J, Pypaert M, Shorter J, Warren G. A direct role for GRASP65 as a mitotically regulated Golgi stacking factor. EMBO J. 2003 Jul 1;22(13):3279-90. PMID:12839990 doi:http://dx.doi.org/10.1093/emboj/cdg317
↑ Feng Y, Yu W, Li X, Lin S, Zhou Y, Hu J, Liu X. Structural insight into Golgi membrane stacking by GRASP65 and GRASP55 proteins. J Biol Chem. 2013 Sep 27;288(39):28418-27. doi: 10.1074/jbc.M113.478024. Epub, 2013 Aug 12. PMID:23940043 doi:http://dx.doi.org/10.1074/jbc.M113.478024
↑ Barr FA, Puype M, Vandekerckhove J, Warren G. GRASP65, a protein involved in the stacking of Golgi cisternae. Cell. 1997 Oct 17;91(2):253-62. PMID:9346242
↑ Feng Y, Yu W, Li X, Lin S, Zhou Y, Hu J, Liu X. Structural insight into Golgi membrane stacking by GRASP65 and GRASP55 proteins. J Biol Chem. 2013 Sep 27;288(39):28418-27. doi: 10.1074/jbc.M113.478024. Epub, 2013 Aug 12. PMID:23940043 doi:http://dx.doi.org/10.1074/jbc.M113.478024

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4kfv"

Categories: Large Structures | Rattus norvegicus | Hu J | Liu X

@@ Line 1: / Line 1: @@
 ==Structural insight into Golgi membrane stacking by GRASP65 and GRASP55==
-<StructureSection load='4kfv' size='340' side='right' caption='[[4kfv]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+<StructureSection load='4kfv' size='340' side='right'caption='[[4kfv]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4kfv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KFV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KFV FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4kfv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KFV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KFV FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4kfw|4kfw]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kfv OCA], [https://pdbe.org/4kfv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kfv RCSB], [https://www.ebi.ac.uk/pdbsum/4kfv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kfv ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Gorasp1, GRASP 65, rCG_25352 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kfv OCA], [http://pdbe.org/4kfv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4kfv RCSB], [http://www.ebi.ac.uk/pdbsum/4kfv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4kfv ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/GORS1_RAT GORS1_RAT] Key structural protein of the Golgi apparatus (By similarity). The membrane cisternae of the Golgi apparatus adhere to each other to form stacks, which are aligned side by side to form the Golgi ribbon (By similarity). Acting in concert with GORASP2/GRASP55, is required for the formation and maintenance of the Golgi ribbon, and may be dispensable for the formation of stacks (By similarity). However, other studies suggest that GORASP1 plays an important role in assembly and membrane stacking of the cisternae, and in the reassembly of Golgi stacks after breakdown during mitosis (PubMed:12839990, PubMed:23940043, PubMed:9346242). Caspase-mediated cleavage of GORASP1 is required for fragmentation of the Golgi during apoptosis (PubMed:11815631, PubMed:12015985). Also mediates, via its interaction with GOLGA2/GM130, the docking of transport vesicles with the Golgi membranes (By similarity). Mediates ER stress-induced unconventional (ER/Golgi-independent) trafficking of core-glycosylated CFTR to cell membrane (By similarity).[UniProtKB:Q91X51][UniProtKB:Q9BQQ3]<ref>PMID:11815631</ref> <ref>PMID:12015985</ref> <ref>PMID:12839990</ref> <ref>PMID:23940043</ref> <ref>PMID:9346242</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Buffalo rat]]
+[[Category: Large Structures]]
-[[Category: Hu, J]]
+[[Category: Rattus norvegicus]]
-[[Category: Liu, X]]
+[[Category: Hu J]]
-[[Category: Golgi]]
+[[Category: Liu X]]
-[[Category: Golgi stacking protein]]
-[[Category: Pdz domain]]
-[[Category: Signaling protein]]

4kfv

From Proteopedia

Revision as of 11:56, 30 November 2022

Structural insight into Golgi membrane stacking by GRASP65 and GRASP55

Structural highlights

Function

Publication Abstract from PubMed

References

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