1nsn

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1nsn.gif|left|200px]]
[[Image:1nsn.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1nsn |SIZE=350|CAPTION= <scene name='initialview01'>1nsn</scene>, resolution 2.8&Aring;
+
The line below this paragraph, containing "STRUCTURE_1nsn", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND=
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Micrococcal_nuclease Micrococcal nuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.31.1 3.1.31.1] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE= NUCLEASE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1nsn| PDB=1nsn | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nsn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nsn OCA], [http://www.ebi.ac.uk/pdbsum/1nsn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1nsn RCSB]</span>
+
-
}}
+
'''THE CRYSTAL STRUCTURE OF ANTIBODY N10-STAPHYLOCOCCAL NUCLEASE COMPLEX AT 2.9 ANGSTROMS RESOLUTION'''
'''THE CRYSTAL STRUCTURE OF ANTIBODY N10-STAPHYLOCOCCAL NUCLEASE COMPLEX AT 2.9 ANGSTROMS RESOLUTION'''
Line 29: Line 26:
[[Category: Bossart-Whitaker, P.]]
[[Category: Bossart-Whitaker, P.]]
[[Category: Sheriff, S.]]
[[Category: Sheriff, S.]]
-
[[Category: immunoglobulin]]
+
[[Category: Immunoglobulin]]
-
[[Category: staphylococcal nuclease]]
+
[[Category: Staphylococcal nuclease]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:55:51 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:34:55 2008''
+

Revision as of 23:55, 2 May 2008

Image:1nsn.gif

Template:STRUCTURE 1nsn

THE CRYSTAL STRUCTURE OF ANTIBODY N10-STAPHYLOCOCCAL NUCLEASE COMPLEX AT 2.9 ANGSTROMS RESOLUTION


Overview

The three-dimensional structure of the antibody N10 Fab fragment complexed with staphylococcal nuclease (SNase) has been determined to 2.9 A resolution. Eighteen residues from six complementarity-determining regions (CDR) recognize an epitope of five distinct SNase segments with a total of 17 residues. The overall shape of the antibody-antigen interface is U-shaped rather than the more or less rectangular interface seen in other antibody-protein antigen interfaces. Despite the U-shaped interface, the amount of surface buried in the complex, 828 A2 for SNase and 793 A2 for N10, is typical of antibody-protein antigen complexes. Contributing to the shape of the interface is the shortest antibody heavy chain-CDR3 loop reported to date, which probably allows access of bulk solvent in the center of the "U" interface. Another unusual feature of the N10 antibody is the 15 residue antibody light chain-CDR1, a length seen in only three other reported antibodies. Antibody light chain-CDR1 displays a previously unobserved conformation in its distal portion. Finally, although some of the movement observed in the antibody-bound SNase may be due to crystal contacts, it is clear that some side-chain rearrangements are the result of antigen-antibody interaction.

About this Structure

1NSN is a Protein complex structure of sequences from Mus musculus and Staphylococcus aureus. Full crystallographic information is available from OCA.

Reference

The crystal structure of the antibody N10-staphylococcal nuclease complex at 2.9 A resolution., Bossart-Whitaker P, Chang CY, Novotny J, Benjamin DC, Sheriff S, J Mol Biol. 1995 Nov 3;253(4):559-75. PMID:7473734 Page seeded by OCA on Sat May 3 02:55:51 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1nsn"
Personal tools