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1nsy

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[[Image:1nsy.gif|left|200px]]
[[Image:1nsy.gif|left|200px]]
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{{Structure
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|PDB= 1nsy |SIZE=350|CAPTION= <scene name='initialview01'>1nsy</scene>, resolution 2.0&Aring;
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The line below this paragraph, containing "STRUCTURE_1nsy", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=ATP:ADENOSINE-5&#39;-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(+)_synthase_(glutamine-hydrolyzing) NAD(+) synthase (glutamine-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.5.1 6.3.5.1] </span>
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{{STRUCTURE_1nsy| PDB=1nsy | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nsy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nsy OCA], [http://www.ebi.ac.uk/pdbsum/1nsy PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1nsy RCSB]</span>
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'''CRYSTAL STRUCTURE OF NH3-DEPENDENT NAD+ SYNTHETASE FROM BACILLUS SUBTILIS'''
'''CRYSTAL STRUCTURE OF NH3-DEPENDENT NAD+ SYNTHETASE FROM BACILLUS SUBTILIS'''
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Crystal structure of NH3-dependent NAD+ synthetase from Bacillus subtilis., Rizzi M, Nessi C, Mattevi A, Coda A, Bolognesi M, Galizzi A, EMBO J. 1996 Oct 1;15(19):5125-34. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8895556 8895556]
Crystal structure of NH3-dependent NAD+ synthetase from Bacillus subtilis., Rizzi M, Nessi C, Mattevi A, Coda A, Bolognesi M, Galizzi A, EMBO J. 1996 Oct 1;15(19):5125-34. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8895556 8895556]
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: NAD(+) synthase (glutamine-hydrolyzing)]]
 
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Bolognesi, M.]]
[[Category: Bolognesi, M.]]
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[[Category: Nessi, C.]]
[[Category: Nessi, C.]]
[[Category: Rizzi, M.]]
[[Category: Rizzi, M.]]
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[[Category: amidotransferase]]
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[[Category: Amidotransferase]]
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[[Category: atp pyrophosphatase]]
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[[Category: Atp pyrophosphatase]]
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[[Category: lyase]]
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[[Category: Lyase]]
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[[Category: nh3 dependent]]
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[[Category: Nh3 dependent]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:56:25 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:35:07 2008''
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Revision as of 23:56, 2 May 2008

Image:1nsy.gif

Template:STRUCTURE 1nsy

CRYSTAL STRUCTURE OF NH3-DEPENDENT NAD+ SYNTHETASE FROM BACILLUS SUBTILIS


Overview

NAD+ synthetase catalyzes the last step in the biosynthesis of nicotinamide adenine dinucleotide. The three-dimensional structure of NH3-dependent NAD+ synthetase from Bacillus subtilis, in its free form and in complex with ATP, has been solved by X-ray crystallography (at 2.6 and 2.0 angstroms resolution, respectively) using a combination of multiple isomorphous replacement and density modification techniques. The enzyme consists of a tight homodimer with alpha/beta subunit topology. The catalytic site is located at the parallel beta-sheet topological switch point, where one AMP molecule, one pyrophosphate and one Mg2+ ion are observed. Residue Ser46, part of the neighboring 'P-loop', is hydrogen bonded to the pyrophosphate group, and may play a role in promoting the adenylation of deamido-NAD+ during the first step of the catalyzed reaction. The deamido-NAD+ binding site, located at the subunit interface, is occupied by one ATP molecule, pointing towards the catalytic center. A conserved structural fingerprint of the catalytic site, comprising Ser46, is very reminiscent of a related protein region observed in glutamine-dependent GMP synthetase, supporting the hypothesis that NAD+ synthetase belongs to the newly discovered family of 'N-type' ATP pyrophosphatases.

About this Structure

1NSY is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

Crystal structure of NH3-dependent NAD+ synthetase from Bacillus subtilis., Rizzi M, Nessi C, Mattevi A, Coda A, Bolognesi M, Galizzi A, EMBO J. 1996 Oct 1;15(19):5125-34. PMID:8895556 Page seeded by OCA on Sat May 3 02:56:25 2008

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