1ntb
From Proteopedia
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[[Image:1ntb.gif|left|200px]] | [[Image:1ntb.gif|left|200px]] | ||
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'''2.9 A crystal structure of Streptomycin RNA-aptamer complex''' | '''2.9 A crystal structure of Streptomycin RNA-aptamer complex''' | ||
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==About this Structure== | ==About this Structure== | ||
- | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NTB OCA]. | |
==Reference== | ==Reference== | ||
Encapsulating streptomycin within a small 40-mer RNA., Tereshko V, Skripkin E, Patel DJ, Chem Biol. 2003 Feb;10(2):175-87. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12618190 12618190] | Encapsulating streptomycin within a small 40-mer RNA., Tereshko V, Skripkin E, Patel DJ, Chem Biol. 2003 Feb;10(2):175-87. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12618190 12618190] | ||
- | [[Category: Protein complex]] | ||
[[Category: Patel, D J.]] | [[Category: Patel, D J.]] | ||
[[Category: Skripkin, E.]] | [[Category: Skripkin, E.]] | ||
[[Category: Tereshko, V.]] | [[Category: Tereshko, V.]] | ||
- | [[Category: | + | [[Category: Magnesium form]] |
- | [[Category: | + | [[Category: Streptomycin rna-aptamer]] |
- | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:57:16 2008'' | |
- | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + |
Revision as of 23:57, 2 May 2008
2.9 A crystal structure of Streptomycin RNA-aptamer complex
Overview
We describe a 2.9 A X-ray structure of a complex between the aminocyclitol antibiotic streptomycin and an in vitro selected RNA aptamer, solved using the anomalous diffraction properties of Ba cations. The RNA aptamer, which contains two asymmetric internal loops, adopts a distinct cation-stabilized fold involving a series of S-shaped backbone turns anchored by canonical and noncanonical pairs and triples. The streptomycin streptose ring is encapsulated by stacked arrays of bases from both loops at the elbow of the L-shaped RNA architecture. Specificity is defined by direct hydrogen bonds between all streptose functional groups and base edges that line the inner walls of the cylindrical binding pocket. By contrast, the majority of intermolecular interactions involve contacts to backbone phosphates in the published structure of streptomycin bound to the 16S rRNA.
About this Structure
Full crystallographic information is available from OCA.
Reference
Encapsulating streptomycin within a small 40-mer RNA., Tereshko V, Skripkin E, Patel DJ, Chem Biol. 2003 Feb;10(2):175-87. PMID:12618190 Page seeded by OCA on Sat May 3 02:57:16 2008