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1nth
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'''Crystal structure of the methanosarcina barkeri monomethylamine methyltransferase (MTMB)''' | '''Crystal structure of the methanosarcina barkeri monomethylamine methyltransferase (MTMB)''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1NTH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methanosarcina_barkeri Methanosarcina barkeri]. This structure supersedes the now removed PDB entry | + | 1NTH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methanosarcina_barkeri Methanosarcina barkeri]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1l2r 1l2r]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NTH OCA]. |
==Reference== | ==Reference== | ||
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[[Category: James, C M.]] | [[Category: James, C M.]] | ||
[[Category: Krzycki, J A.]] | [[Category: Krzycki, J A.]] | ||
| - | [[Category: | + | [[Category: Tim barrel]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:57:32 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 23:57, 2 May 2008
Crystal structure of the methanosarcina barkeri monomethylamine methyltransferase (MTMB)
Overview
Genes encoding methanogenic methylamine methyltransferases all contain an in-frame amber (UAG) codon that is read through during translation. We have identified the UAG-encoded residue in a 1.55 angstrom resolution structure of the Methanosarcina barkeri monomethylamine methyltransferase (MtmB). This structure reveals a homohexamer comprised of individual subunits with a TIM barrel fold. The electron density for the UAG-encoded residue is distinct from any of the 21 natural amino acids. Instead it appears consistent with a lysine in amide-linkage to (4R,5R)-4-substituted-pyrroline-5-carboxylate. We suggest that this amino acid be named l-pyrrolysine.
About this Structure
1NTH is a Single protein structure of sequence from Methanosarcina barkeri. This structure supersedes the now removed PDB entry 1l2r. Full crystallographic information is available from OCA.
Reference
A new UAG-encoded residue in the structure of a methanogen methyltransferase., Hao B, Gong W, Ferguson TK, James CM, Krzycki JA, Chan MK, Science. 2002 May 24;296(5572):1462-6. PMID:12029132 Page seeded by OCA on Sat May 3 02:57:32 2008
