1nui
From Proteopedia
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'''Crystal Structure of the primase fragment of Bacteriophage T7 primase-helicase protein''' | '''Crystal Structure of the primase fragment of Bacteriophage T7 primase-helicase protein''' | ||
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[[Category: Richardson, C C.]] | [[Category: Richardson, C C.]] | ||
[[Category: Wagner, G.]] | [[Category: Wagner, G.]] | ||
| - | [[Category: | + | [[Category: Atp-binding]] |
| - | [[Category: | + | [[Category: Dna replication]] |
| - | [[Category: | + | [[Category: Dna-directed rna polymerase]] |
| - | [[Category: | + | [[Category: Late protein]] |
| - | [[Category: | + | [[Category: Primosome]] |
| - | [[Category: | + | [[Category: Toprim fold]] |
| - | [[Category: | + | [[Category: Transferase]] |
| - | [[Category: | + | [[Category: Zinc-biding domain]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:59:44 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 23:59, 2 May 2008
Crystal Structure of the primase fragment of Bacteriophage T7 primase-helicase protein
Overview
DNA primases are template-dependent RNA polymerases that synthesize oligoribonucleotide primers that can be extended by DNA polymerase. The bacterial primases consist of zinc binding and RNA polymerase domains that polymerize ribonucleotides at templating sequences of single-stranded DNA. We report a crystal structure of bacteriophage T7 primase that reveals its two domains and the presence of two Mg(2+) ions bound to the active site. NMR and biochemical data show that the two domains remain separated until the primase binds to DNA and nucleotide. The zinc binding domain alone can stimulate primer extension by T7 DNA polymerase. These findings suggest that the zinc binding domain couples primer synthesis with primer utilization by securing the DNA template in the primase active site and then delivering the primed DNA template to DNA polymerase. The modular architecture of the primase and a similar mechanism of priming DNA synthesis are likely to apply broadly to prokaryotic primases.
About this Structure
1NUI is a Single protein structure of sequence from Enterobacteria phage t7. Full crystallographic information is available from OCA.
Reference
Modular architecture of the bacteriophage T7 primase couples RNA primer synthesis to DNA synthesis., Kato M, Ito T, Wagner G, Richardson CC, Ellenberger T, Mol Cell. 2003 May;11(5):1349-60. PMID:12769857 Page seeded by OCA on Sat May 3 02:59:44 2008
