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| | ==Vaccinia D8L ectodomain structure== | | ==Vaccinia D8L ectodomain structure== |
| - | <StructureSection load='4e9o' size='340' side='right' caption='[[4e9o]], [[Resolution|resolution]] 1.42Å' scene=''> | + | <StructureSection load='4e9o' size='340' side='right'caption='[[4e9o]], [[Resolution|resolution]] 1.42Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4e9o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E9O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E9O FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4e9o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E9O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E9O FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ypy|1ypy]], [[2i9l|2i9l]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e9o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e9o OCA], [https://pdbe.org/4e9o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e9o RCSB], [https://www.ebi.ac.uk/pdbsum/4e9o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e9o ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VACAC2_124, VACCL3_124, VAC_DPP17_124 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10245 Vaccinia virus])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e9o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e9o OCA], [http://pdbe.org/4e9o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4e9o RCSB], [http://www.ebi.ac.uk/pdbsum/4e9o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4e9o ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [[https://www.uniprot.org/uniprot/Q1M1K6_9POXV Q1M1K6_9POXV]] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Large Structures]] |
| | [[Category: Vaccinia virus]] | | [[Category: Vaccinia virus]] |
| - | [[Category: Matho, M H]] | + | [[Category: Matho MH]] |
| - | [[Category: Zajonc, D M]] | + | [[Category: Zajonc DM]] |
| - | [[Category: Beta sheet]]
| + | |
| - | [[Category: Cah alpha fold]]
| + | |
| - | [[Category: Cell surface chondroitin binding]]
| + | |
| - | [[Category: Chondroitin sulfate]]
| + | |
| - | [[Category: Viral entry]]
| + | |
| - | [[Category: Viral protein]]
| + | |
| - | [[Category: Vp7 motif]]
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| Structural highlights
Function
[Q1M1K6_9POXV]
Publication Abstract from PubMed
Smallpox vaccine is considered a gold standard of vaccines, as it is the only one that has led to the complete eradication of an infectious disease from the human population. B cell responses are critical for the protective immunity induced by the vaccine, yet their targeted epitopes recognized in humans remain poorly described. Here we describe the biochemical and structural characterization of one of the immunodominant vaccinia virus (VACV) antigens D8 and its binding to the monoclonal antibody LA5, which is capable of neutralizing VACV in the presence of complement. Full length D8 ectodomain was found to form a tetramer. We determined the crystal structure of the LA5 Fab - monomeric D8 complex at a resolution of 2.1 A, as well as the unliganded structures of D8 and LA5-Fab at resolutions of 1.42 A and 1.6 A, respectively. D8 features a carbonic anhydrase (CAH) fold that has evolved to bind to the glycosaminoglycan (GAG) chondroitin sulfate (CS) on host cells. The central positively charged crevice of D8 was predicted as the CS binding site by automated docking experiments. Furthermore, sequence alignment of various poxvirus D8 orthologs revealed that this crevice is structurally conserved. The D8 epitope is formed by 23 discontinuous residues that are spread across 80% of the D8 protein sequence. Interestingly, LA5 binds with a high affinity lock-and-key mechanism above this crevice with an unusually large antibody - antigen interface, burying 2434 A(2) of protein surface.
Structural and biochemical characterization of the vaccinia virus envelope protein D8 and its recognition by the antibody LA5.,Matho MH, Maybeno M, Benhnia MR, Becker D, Meng X, Xiang Y, Crotty S, Peters B, Zajonc DM J Virol. 2012 May 23. PMID:22623786[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Matho MH, Maybeno M, Benhnia MR, Becker D, Meng X, Xiang Y, Crotty S, Peters B, Zajonc DM. Structural and biochemical characterization of the vaccinia virus envelope protein D8 and its recognition by the antibody LA5. J Virol. 2012 May 23. PMID:22623786 doi:10.1128/JVI.00836-12
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