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3j91

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Revision as of 17:30, 6 March 2020

Cryo-electron microscopy of Enterovirus 71 (EV71) procapsid in complex with Fab fragments of neutralizing antibody 22A12

3j91, resolution 8.80Å

Structural highlights

3j91 is a 2 chain structure with sequence from Enterovirus a71. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[E5RPG0_9ENTO] Protein 2C associates with and induces structural rearrangements of intracellular membranes. It displays RNA-binding, nucleotide binding and NTPase activities (By similarity).[SAAS:SAAS001676_004_016611] Protein 3C is a cysteine protease that generates mature viral proteins from the precursor polyprotein. In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind co-operatively to the protease (By similarity).[SAAS:SAAS000199_004_042266] RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals (By similarity).[SAAS:SAAS001676_004_010047]

Publication Abstract from PubMed

Enterovirus 71 (EV71) is responsible for seasonal outbreaks of hand, foot, and mouth disease in the Asia-Pacific region. The virus has the capability of causing severe disease and death, especially in young children. Although several vaccines are currently in clinical trials no vaccines or therapeutics have been approved for use. Previous structural studies have revealed that two antigenically distinct capsid forms are produced in EV71 infected cells: an expanded empty capsid, sometimes called procapsid, and the infectious virus. Specifically an immunodominant epitope of EV71 that maps to the virus canyon is structurally different between the procapsid and virus. This structure function study shows that the procapsid can sequester antibodies thus enhancing EV71 infection in vitro. The results presented here suggest that due to conformational differences between the EV71 procapsid and virus, the presence of the procapsid in natural virus infections should be considered in the future design of vaccines or therapeutics. IMPORTANCE: In a picornavirus infection both an infectious and a non-infectious empty capsid, sometimes referred to as procapsid, are produced. It was novel to discover that the procapsid form of enterovirus 71 (EV71) was expanded and antigenically distinct from the infectious virus. Previously it had been supposed that this empty capsid was an off pathway dead end, or at best served as storage of pentameric subunits, which was later shown to be unlikely. It remains unexplained why picornaviruses evolutionarily conserve the wasteful production of so much non-infectious capsid. Here we demonstrate that the EV71 procapsid has different antigenic properties than the infectious virus. Thus the procapsid has the capacity to sequester neutralizing antibody and protect the virus, promoting or restoring a successful infection in vitro. This important observation should be considered in the future design and development of vaccines and therapeutics.

The Enterovirus 71 procapsid binds neutralizing antibodies and rescues virus infection in vitro.,Shingler KL, Cifuente JO, Ashley RE, Makhov AM, Conway JF, Hafenstein S J Virol. 2014 Nov 26. pii: JVI.03098-14. PMID:25428877^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Shingler KL, Cifuente JO, Ashley RE, Makhov AM, Conway JF, Hafenstein S. The Enterovirus 71 procapsid binds neutralizing antibodies and rescues virus infection in vitro. J Virol. 2014 Nov 26. pii: JVI.03098-14. PMID:25428877 doi:http://dx.doi.org/10.1128/JVI.03098-14

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3j91"

@@ Line 1: / Line 1: @@
 ==Cryo-electron microscopy of Enterovirus 71 (EV71) procapsid in complex with Fab fragments of neutralizing antibody 22A12==
-<StructureSection load='3j91' size='340' side='right' caption='[[3j91]], [[Resolution|resolution]] 8.80&Aring;' scene=''>
+<SX load='3j91' size='340' side='right' viewer='molstar' caption='[[3j91]], [[Resolution|resolution]] 8.80&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[3j91]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterovirus_a71 Enterovirus a71]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J91 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J91 FirstGlance]. <br>
@@ Line 20: / Line 20: @@
 <references/>
 __TOC__
-</StructureSection>
+</SX>
 [[Category: Enterovirus a71]]
+[[Category: Large Structures]]
 [[Category: Ashley, R E]]
 [[Category: Cifuente, J O]]

3j91

From Proteopedia

Revision as of 17:30, 6 March 2020

Cryo-electron microscopy of Enterovirus 71 (EV71) procapsid in complex with Fab fragments of neutralizing antibody 22A12

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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