1o8c
From Proteopedia
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'''CRYSTAL STRUCTURE OF E. COLI K-12 YHDH WITH BOUND NADPH''' | '''CRYSTAL STRUCTURE OF E. COLI K-12 YHDH WITH BOUND NADPH''' | ||
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[[Category: Valencia, C.]] | [[Category: Valencia, C.]] | ||
[[Category: Vincentelli, R.]] | [[Category: Vincentelli, R.]] | ||
| - | [[Category: | + | [[Category: Possible nadph-dependent quinone oxidoreductase]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:30:49 2008'' | |
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Revision as of 00:30, 3 May 2008
CRYSTAL STRUCTURE OF E. COLI K-12 YHDH WITH BOUND NADPH
Overview
As part of a structural genomics project on bacterial gene products of unknown function, the crystal structures of YhdH, a putative quinone oxidoreductase, and its complex with NADP have been determined at 2.25 and 2.6 A resolution, respectively. The overall fold of YhdH is very similar to that of alcohol dehydrogenases and quinone reductases despite its low sequence identity. The absence of any Zn ion indicates that YdhH is a putative quinone oxidoreductase. YhdH forms a homodimer, with each subunit composed of two domains: a catalytic domain and a coenzyme-binding domain. NADP is bound in a deep cleft formed between the two domains. Large conformational changes occur upon NADP binding, with the two domains closing up to each other and narrowing the NADP-binding cleft. Comparisons of the YdhH active site with those of the quinone oxidoreductases from Escherichia coli and Thermus thermophilus made it possible to identify essential conserved residues as being Asn41, Asp43, Asp64 and Arg318. The active-site size is very narrow and unless an induced fit occurs is accessible only to reagents the size of benzoquinone.
About this Structure
1O8C is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structure of Escherichia coli YhdH, a putative quinone oxidoreductase., Sulzenbacher G, Roig-Zamboni V, Pagot F, Grisel S, Salomoni A, Valencia C, Campanacci V, Vincentelli R, Tegoni M, Eklund H, Cambillau C, Acta Crystallogr D Biol Crystallogr. 2004 Oct;60(Pt 10):1855-62. Epub 2004, Sep 23. PMID:15388933 Page seeded by OCA on Sat May 3 03:30:49 2008
