Serine palmitoyltransferase
From Proteopedia
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| - | + | <StructureSection load='3a2b' size='450' side='right' scene='' caption='Monomer of the dimeric prokaryotic serine palmitoyltransferase complex with serine and cofactor pyridoxal phosphate [[3a2b]]'> | |
== Function == | == Function == | ||
'''Serine palmitoyltransferase''' (SPT) catalyzes the conversion of palmitoyl-CoA and serine to CoA and dehydro-sphinganine. This reaction is part of sphingosine biosynthesis. Pyridoxal phosphate (PLP) is a cofactor in the reaction. The prokaryotic SPT is a soluble homodimer while the eukaryotic one is heterodimeric and is anchored in the endoplasmic reticulum<ref>PMID:12782147</ref>. | '''Serine palmitoyltransferase''' (SPT) catalyzes the conversion of palmitoyl-CoA and serine to CoA and dehydro-sphinganine. This reaction is part of sphingosine biosynthesis. Pyridoxal phosphate (PLP) is a cofactor in the reaction. The prokaryotic SPT is a soluble homodimer while the eukaryotic one is heterodimeric and is anchored in the endoplasmic reticulum<ref>PMID:12782147</ref>. | ||
== Structural highlights == | == Structural highlights == | ||
| - | The active site of SPT contains a | + | The active site of SPT contains a serine residue covalently bound to the cofactor PLP<ref>PMID:19564159</ref>. |
| - | + | </StructureSection> | |
==3D structures of serine palmitoyltransferase== | ==3D structures of serine palmitoyltransferase== | ||
Revision as of 12:05, 25 August 2016
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3D structures of serine palmitoyltransferase
Updated on 25-August-2016
2jg2 – PpSPT + aldimine – Pseudomonas paucimobilis
2jgt – PpSPT
2w8j - SpSPT + aldimine – Sphingomonas paucimobilis
2w8t, 2w8u, 2w8v, 2w8w - SpSPT (mutant) + aldimine
4bmk - SpSPT (mutant) + myriocin + PLP
2xbn – SpSPT + pyridoxamine phosphate
3a2b – SPT + pyridoxal phosphate + serine – Sphingobacterium multivorum
2x8u - SwSPT + pyridoxal derivative – Sphingomonas wittichii
References
- ↑ Hanada K. Serine palmitoyltransferase, a key enzyme of sphingolipid metabolism. Biochim Biophys Acta. 2003 Jun 10;1632(1-3):16-30. PMID:12782147
- ↑ Ikushiro H, Islam MM, Okamoto A, Hoseki J, Murakawa T, Fujii S, Miyahara I, Hayashi H. Structural insights into the enzymatic mechanism of serine palmitoyltransferase from Sphingobacterium multivorum. J Biochem. 2009 Oct;146(4):549-62. Epub 2009 Jun 29. PMID:19564159 doi:10.1093/jb/mvp100
