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| | + | '''Unreleased structure''' |
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| - | ==Crystal structure of KAI2-like protein from Striga (apo state 1)==
| + | The entry 5dnw is ON HOLD until Paper Publication |
| - | <StructureSection load='5dnw' size='340' side='right' caption='[[5dnw]], [[Resolution|resolution]] 2.02Å' scene=''>
| + | |
| - | == Structural highlights ==
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| - | <table><tr><td colspan='2'>[[5dnw]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DNW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DNW FirstGlance]. <br>
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| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5dnu|5dnu]], [[5dnv|5dnv]]</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dnw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dnw OCA], [http://pdbe.org/5dnw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dnw RCSB], [http://www.ebi.ac.uk/pdbsum/5dnw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5dnw ProSAT]</span></td></tr>
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| - | </table>
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| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | The perception of two plant germination inducers, karrikins and strigolactones, are mediated by the proteins KAI2 and D14. Recently, KAI2-type proteins from parasitic weeds, which are possibly related to seed germination induced by strigolactone, have been classified into three clades characterized by different responses to karrikin/strigolactone. Here we characterized a karrikin-binding protein in Striga (ShKAI2iB) that belongs to intermediate-evolving KAI2 and provided the structural bases for its karrikin-binding specificity. Binding assays showed that ShKAI2iB bound karrikins but not strigolactone, differing from other KAI2 and D14. The crystal structures of ShKAI2iB and ShKAI2iB-karrikin complex revealed obvious structural differences in a helix located at the entry of its ligand-binding cavity. This results in a smaller closed pocket, which is also the major cause of ShKAI2iB's specificity of binding karrikin. Our structural study also revealed that a few non-conserved amino acids led to the distinct ligand-binding profile of ShKAI2iB, suggesting that the evolution of KAI2 resulted in its diverse functions.
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| - | Structural basis of unique ligand specificity of KAI2-like protein from parasitic weed Striga hermonthica.,Xu Y, Miyakawa T, Nakamura H, Nakamura A, Imamura Y, Asami T, Tanokura M Sci Rep. 2016 Aug 10;6:31386. doi: 10.1038/srep31386. PMID:27507097<ref>PMID:27507097</ref>
| + | Authors: |
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | Description: |
| - | </div>
| + | [[Category: Unreleased Structures]] |
| - | <div class="pdbe-citations 5dnw" style="background-color:#fffaf0;"></div>
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| - | == References ==
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| - | <references/>
| + | |
| - | __TOC__
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| - | </StructureSection>
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| - | [[Category: Miyakawa, T]]
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| - | [[Category: Nakamura, A]]
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| - | [[Category: Tanokura, M]]
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| - | [[Category: Xu, Y]]
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| - | [[Category: Hydrolase]]
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| - | [[Category: Kai2 karrikin striga]] | + | |
