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5j8e

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Revision as of 10:03, 22 November 2017

Crystal structure of human Hook3's conserved Hook domain

Structural highlights

5j8e is a 2 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	HOOK3 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[HOOK3_HUMAN] Probably serves as a target for the spiC protein from Salmonella typhimurium, which inactivates it, leading to a strong alteration in cellular trafficking (By similarity). Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). May regulate clearance of endocytosed receptors such as MSR1. Participates in defining the architecture and localization of the Golgi complex.^[1] ^[2] ^[3]

Publication Abstract from PubMed

Metazoan cytoplasmic dynein moves processively along microtubules with the aid of dynactin and an adaptor protein that joins dynein and dynactin into a stable ternary complex. Here, we examined how Hook3, a cargo adaptor involved in Golgi and endosome transport, forms a motile dynein-dynactin complex. We show that the conserved Hook domain interacts directly with the dynein light intermediate chain 1 (LIC1). By solving the crystal structure of the Hook domain and using structure-based mutagenesis, we identify two conserved surface residues that are each critical for LIC1 binding. Hook proteins with mutations in these residues fail to form a stable dynein-dynactin complex, revealing a crucial role for LIC1 in this interaction. We also identify a region of Hook3 specifically required for an allosteric activation of processive motility. Our work reveals the structural details of Hook3's interaction with dynein and offers insight into how cargo adaptors form processive dynein-dynactin motor complexes.

Assembly and activation of dynein-dynactin by the cargo adaptor protein Hook3.,Schroeder CM, Vale RD J Cell Biol. 2016 Aug 1;214(3):309-18. doi: 10.1083/jcb.201604002. PMID:27482052^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Walenta JH, Didier AJ, Liu X, Kramer H. The Golgi-associated hook3 protein is a member of a novel family of microtubule-binding proteins. J Cell Biol. 2001 Mar 5;152(5):923-34. PMID:11238449
↑ Sano H, Ishino M, Kramer H, Shimizu T, Mitsuzawa H, Nishitani C, Kuroki Y. The microtubule-binding protein Hook3 interacts with a cytoplasmic domain of scavenger receptor A. J Biol Chem. 2007 Mar 16;282(11):7973-81. Epub 2007 Jan 19. PMID:17237231 doi:http://dx.doi.org/10.1074/jbc.M611537200
↑ Xu L, Sowa ME, Chen J, Li X, Gygi SP, Harper JW. An FTS/Hook/p107(FHIP) complex interacts with and promotes endosomal clustering by the homotypic vacuolar protein sorting complex. Mol Biol Cell. 2008 Dec;19(12):5059-71. Epub 2008 Sep 17. PMID:18799622 doi:http://dx.doi.org/E08-05-0473
↑ Schroeder CM, Vale RD. Assembly and activation of dynein-dynactin by the cargo adaptor protein Hook3. J Cell Biol. 2016 Aug 1;214(3):309-18. doi: 10.1083/jcb.201604002. PMID:27482052 doi:http://dx.doi.org/10.1083/jcb.201604002

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5j8e"

@@ Line 3: / Line 3: @@
 <StructureSection load='5j8e' size='340' side='right' caption='[[5j8e]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5j8e]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J8E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5J8E FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5j8e]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J8E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5J8E FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5j8e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j8e OCA], [http://pdbe.org/5j8e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j8e RCSB], [http://www.ebi.ac.uk/pdbsum/5j8e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j8e ProSAT]</span></td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HOOK3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5j8e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j8e OCA], [http://pdbe.org/5j8e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j8e RCSB], [http://www.ebi.ac.uk/pdbsum/5j8e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j8e ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Human]]
 [[Category: Ekiert, D C]]
 [[Category: Schroeder, C M]]

5j8e

From Proteopedia

Revision as of 10:03, 22 November 2017

Crystal structure of human Hook3's conserved Hook domain

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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