1of9
From Proteopedia
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'''SOLUTION STRUCTURE OF THE PORE FORMING TOXIN OF ENTAMOEBA HISTOLYTICA (AMOEBAPORE A)''' | '''SOLUTION STRUCTURE OF THE PORE FORMING TOXIN OF ENTAMOEBA HISTOLYTICA (AMOEBAPORE A)''' | ||
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[[Category: Nuland, N Van.]] | [[Category: Nuland, N Van.]] | ||
[[Category: Schleinkofer, K.]] | [[Category: Schleinkofer, K.]] | ||
| - | [[Category: | + | [[Category: Amoebapore some]] |
| - | [[Category: | + | [[Category: Pore forming]] |
| - | [[Category: | + | [[Category: Saplip]] |
| - | [[Category: | + | [[Category: Toxin]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:46:19 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 00:46, 3 May 2008
SOLUTION STRUCTURE OF THE PORE FORMING TOXIN OF ENTAMOEBA HISTOLYTICA (AMOEBAPORE A)
Overview
Amoebapore A is a 77-residue protein from the protozoan parasite and human pathogen Entamoeba histolytica. Amoebapores lyse both bacteria and eukaryotic cells by pore formation and play a pivotal role in the destruction of host tissues during amoebiasis, one of the most life-threatening parasitic diseases. Amoebapore A belongs to the superfamily of saposin-like proteins that are characterized by a conserved disulfide bond pattern and a fold consisting of five helices. Membrane-permeabilizing effector molecules of mammalian lymphocytes such as porcine NK-lysin and the human granulysin share these structural attributes. Several mechanisms have been proposed to explain how saposin-like proteins form membrane pores. All mechanisms indicate that the surface charge distribution of these proteins is the basis of their membrane binding capacity and pore formation. Here, we have solved the structure of amoebapore A by NMR spectroscopy. We demonstrate that the specific activation step of amoebapore A depends on a pH-dependent dimerization event and is modulated by a surface-exposed histidine residue. Thus, histidine-mediated dimerization is the molecular switch for pore formation and reveals a novel activation mechanism of pore-forming toxins.
About this Structure
1OF9 is a Single protein structure of sequence from Entamoeba histolytica. Full crystallographic information is available from OCA.
Reference
Solution structure of the pore-forming protein of Entamoeba histolytica., Hecht O, Van Nuland NA, Schleinkofer K, Dingley AJ, Bruhn H, Leippe M, Grotzinger J, J Biol Chem. 2004 Apr 23;279(17):17834-41. Epub 2004 Feb 17. PMID:14970207 Page seeded by OCA on Sat May 3 03:46:19 2008
