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1oio
From Proteopedia
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[[Image:1oio.jpg|left|200px]] | [[Image:1oio.jpg|left|200px]] | ||
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'''GAFD (F17C-TYPE) FIMBRIAL ADHESIN FROM ESCHERICHIA COLI''' | '''GAFD (F17C-TYPE) FIMBRIAL ADHESIN FROM ESCHERICHIA COLI''' | ||
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[[Category: Tanskanen, J.]] | [[Category: Tanskanen, J.]] | ||
[[Category: Westerlund-Wikstrom, B.]] | [[Category: Westerlund-Wikstrom, B.]] | ||
| - | [[Category: | + | [[Category: Adhesin]] |
| - | [[Category: | + | [[Category: Glcnac binding lectin]] |
| - | [[Category: | + | [[Category: N-acetyl-d-glucosamine binding]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:53:45 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 00:53, 3 May 2008
GAFD (F17C-TYPE) FIMBRIAL ADHESIN FROM ESCHERICHIA COLI
Overview
GafD in Escherichia coli G (F17) fimbriae is associated with diarrheal disease, and the structure of the ligand-binding domain, GafD1-178, has been determined at 1.7A resolution in the presence of the receptor sugar N-acetyl-D-glucosamine. The overall fold is a beta-barrel jelly-roll fold. The ligand-binding site was identified and localized to the side of the molecule. Receptor binding is mediated by side-chain as well main-chain interactions. Ala43-Asn44, Ser116-Thr117 form the sugar acetamide specificity pocket, while Asp88 confers tight binding and Trp109 appears to position the ligand. There is a disulfide bond that rigidifies the acetamide specificity pocket. The three fimbrial lectins, GafD, FimH and PapG share similar beta-barrel folds but display different ligand-binding regions and disulfide-bond patterns. We suggest an evolutionary path for the evolution of the very diverse fimbrial lectins from a common ancestral fold.
About this Structure
1OIO is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
The structural basis of receptor-binding by Escherichia coli associated with diarrhea and septicemia., Merckel MC, Tanskanen J, Edelman S, Westerlund-Wikstrom B, Korhonen TK, Goldman A, J Mol Biol. 2003 Aug 22;331(4):897-905. PMID:12909017 Page seeded by OCA on Sat May 3 03:53:45 2008
