5szt
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the large fragment of DNA Polymerase I from Thermus aquaticus in a closed ternary complex with 7-(N-(10-hydroxydecanoyl)-aminopentenyl)-7-deaza-2'-dATP== | |
| + | <StructureSection load='5szt' size='340' side='right' caption='[[5szt]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5szt]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5SZT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5SZT FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=72J:7-(N-(10-HYDROXYDECANOYL)-AMINOPENTENYL)-7-DEAZA-2-DATP'>72J</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DOC:2,3-DIDEOXYCYTIDINE-5-MONOPHOSPHATE'>DOC</scene></td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5szt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5szt OCA], [http://pdbe.org/5szt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5szt RCSB], [http://www.ebi.ac.uk/pdbsum/5szt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5szt ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Efficient incorporation of modified nucleotides by DNA polymerases is essential for many cutting-edge biomolecular technologies. The present study compares the acceptance of either alkene- or alkyne-modified nucleotides by KlenTaq DNA polymerase and provides structural insights into how 7-deaza-adenosine and thymidine with attached alkene-modifications are incorporated into the growing DNA strand. Thereby, we identified modified nucleotides, which prove to be superior substrates for KlenTaq DNA polymerase compared to their natural analogues. The knowledge can be used to guide future design of functionalized nucleotide building blocks. | ||
| - | + | Structural basis for the KlenTaq DNA Polymerase catalyzed Incorporation of Alkene- versus Alkyne-modified Nucleotides.,Marx A, Hottin A, Betz K, Diederichs K Chemistry. 2016 Nov 30. doi: 10.1002/chem.201604515. PMID:27901305<ref>PMID:27901305</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5szt" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: DNA-directed DNA polymerase]] | ||
[[Category: Betz, K]] | [[Category: Betz, K]] | ||
| - | [[Category: Marx, A]] | ||
[[Category: Hottin, A]] | [[Category: Hottin, A]] | ||
| + | [[Category: Marx, A]] | ||
| + | [[Category: Dna polymerase]] | ||
| + | [[Category: Klentaq]] | ||
| + | [[Category: Linker-modified nucleotide]] | ||
| + | [[Category: Transferase]] | ||
Revision as of 19:06, 15 December 2016
Crystal structure of the large fragment of DNA Polymerase I from Thermus aquaticus in a closed ternary complex with 7-(N-(10-hydroxydecanoyl)-aminopentenyl)-7-deaza-2'-dATP
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