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1e02
From Proteopedia
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| - | [[Image:1e02.gif|left|200px]]<br /> | + | [[Image:1e02.gif|left|200px]]<br /><applet load="1e02" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1e02" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1e02, resolution 2.15Å" /> | caption="1e02, resolution 2.15Å" /> | ||
'''PORCINE ODORANT BINDING PROTEIN COMPLEXED WITH UNDECANAL'''<br /> | '''PORCINE ODORANT BINDING PROTEIN COMPLEXED WITH UNDECANAL'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1E02 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with UNA as [http://en.wikipedia.org/wiki/ligand ligand]. | + | 1E02 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with UNA as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=AC1:Una Binding Site For Chain A'>AC1</scene> and <scene name='pdbsite=AC2:Una Binding Site For Chain B'>AC2</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E02 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: odorant binding protein]] | [[Category: odorant binding protein]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 14:49:51 2007'' |
Revision as of 12:40, 18 December 2007
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PORCINE ODORANT BINDING PROTEIN COMPLEXED WITH UNDECANAL
Overview
Porcine odorant binding protein (pOBP) is a monomer of 157 amino acid, residues, purified in abundance from pig nasal mucosa. In contrast to the, observation on lipocalins as retinol binding protein (RBP), major urinary, protein (MUP) or bovine odorant binding protein (bOBP), no naturally, occurring ligand was found in the beta-barrel cavity of pOBP. Porcine OBP, was therefore chosen as a simple model for structure/function studies with, odorant molecules. In competition experiments with tritiated pyrazine, the, affinity of pOBP towards several odorant molecules belonging to different, chemical classes has been found to be of the micromolar order, with a 1:1, stoichiometry. The X-ray structures of pOBP complexed to these molecules, were determined at resolution between 2.15 and 1.4 A. As expected, the, electron density of the odorant molecules was observed into the, hydrophobic beta-barrel of the lipocalin. Inside this cavity, very few, specific interactions were established between the odorant molecule and, the amino acid side-chains, which did not undergo significant, conformational change. The high B-factors observed for the odorant, molecules as well as the existence of alternative conformations reveal a, non-specific mode of binding of the odorant molecules in the cavity.
About this Structure
1E02 is a Single protein structure of sequence from Sus scrofa with UNA as ligand. Known structural/functional Sites: and . Full crystallographic information is available from OCA.
Reference
Complexes of porcine odorant binding protein with odorant molecules belonging to different chemical classes., Vincent F, Spinelli S, Ramoni R, Grolli S, Pelosi P, Cambillau C, Tegoni M, J Mol Biol. 2000 Jun 30;300(1):127-39. PMID:10864504
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