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5j03

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Revision as of 06:23, 16 October 2019

Crystal Structure of a chimeric Kv7.2 - Kv7.3 proximal C-terminal Domain in Complex with Calmodulin

Structural highlights

5j03 is a 2 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	4v0c, 4umo
Gene:	KCNQ2 (HUMAN), CALM1, CALM, CAM, CAM1, CALM2, CAM2, CAMB, CALM3, CALML2, CAM3, CAMC, CAMIII (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

[KCNQ3_HUMAN] Benign familial infantile epilepsy;Benign familial neonatal seizures;Juvenile myoclonic epilepsy. The disease is caused by mutations affecting the gene represented in this entry. Defects in KCNQ3 may be involved in epileptic disorders. These are characterized by paroxysmal transient disturbances of the electrical activity of the brain that may be manifested as episodic impairment or loss of consciousness, abnormal motor phenomena, psychic or sensory disturbances, or perturbation of the autonomic nervous system.^[1]

Function

[KCNQ3_HUMAN] Probably important in the regulation of neuronal excitability. Associates with KCNQ2 or KCNQ5 to form a potassium channel with essentially identical properties to the channel underlying the native M-current, a slowly activating and deactivating potassium conductance which plays a critical role in determining the subthreshold electrical excitability of neurons as well as the responsiveness to synaptic inputs.

Publication Abstract from PubMed

The Kv7 channel family, comprising voltage-gated potassium channels, plays major roles in fine-tuning cellular excitability by reducing firing frequency and controlling repolarization. Kv7 channels have a unique intracellular C-terminal (CT) domain bound constitutively by calmodulin (CaM). This domain plays key functions in channel tetramerization, trafficking and gating. CaM binds to the proximal CT, comprised of helices A and B. Kv7.2 and Kv7.3 are expressed in neural tissues. Together they form the hetero-tetrameric M channel. We characterized Kv7.2, Kv7.3 and chimeric Kv7.3 helix A-Kv7.2 helix B (Q3A-Q2B) proximal CT/CaM complexes by solution methods at various Ca2+ concentrations and determined them all to have a 1:1 stoichiometry. We then determined the crystal structure of the Q3A-Q2B/CaM complex at high Ca2+ concentration to 2.0 A resolution. CaM hugs the anti-parallel coiled coil of helices A and B, braced together by an additional helix. The structure displays a hybrid apo-Ca2+ CaM conformation even though four Ca2+ ions are bound. Our results pinpoint unique interactions enabling the possible inter-subunit pairing of Kv7.3 helix A and Kv7.2 helix B while underlining the importance of Kv7.3 helix A's role in stabilizing channel oligomerization. Also, the structure can be used to rationalize various channelopathic mutants. Functionally testing of the chimeric channel found it to have a voltage-dependence similar to the M channel, thereby demonstrating helix A's importance in imparting gating properties.

Structural Insights into the M-channel Proximal C-terminus/Calmodulin Complex.,Strulovich R, Tobelaim WS, Attali B, Hirsch JA Biochemistry. 2016 Aug 26. PMID:27564677^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lemke JR, Riesch E, Scheurenbrand T, Schubach M, Wilhelm C, Steiner I, Hansen J, Courage C, Gallati S, Burki S, Strozzi S, Simonetti BG, Grunt S, Steinlin M, Alber M, Wolff M, Klopstock T, Prott EC, Lorenz R, Spaich C, Rona S, Lakshminarasimhan M, Kroll J, Dorn T, Kramer G, Synofzik M, Becker F, Weber YG, Lerche H, Bohm D, Biskup S. Targeted next generation sequencing as a diagnostic tool in epileptic disorders. Epilepsia. 2012 Aug;53(8):1387-98. doi: 10.1111/j.1528-1167.2012.03516.x. Epub, 2012 May 21. PMID:22612257 doi:http://dx.doi.org/10.1111/j.1528-1167.2012.03516.x
↑ Strulovich R, Tobelaim WS, Attali B, Hirsch JA. Structural Insights into the M-channel Proximal C-terminus/Calmodulin Complex. Biochemistry. 2016 Aug 26. PMID:27564677 doi:http://dx.doi.org/10.1021/acs.biochem.6b00477

1 Structural highlights
2 Disease
3 Function
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5j03"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of a chimeric Kv7.2 - Kv7.3 proximal C-terminal Domain in Complex with Calmodulin==
-<StructureSection load='5j03' size='340' side='right' caption='[[5j03]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
+<StructureSection load='5j03' size='340' side='right'caption='[[5j03]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5j03]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J03 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5J03 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5j03]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J03 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5J03 FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4v0c|4v0c]], [[4umo|4umo]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KCNQ2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CALM1, CALM, CAM, CAM1, CALM2, CAM2, CAMB, CALM3, CALML2, CAM3, CAMC, CAMIII ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5j03 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j03 OCA], [http://pdbe.org/5j03 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j03 RCSB], [http://www.ebi.ac.uk/pdbsum/5j03 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j03 ProSAT]</span></td></tr>
 </table>
@@ Line 21: / Line 22: @@
 </div>
 <div class="pdbe-citations 5j03" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Calmodulin 3D structures|Calmodulin 3D structures]]
+*[[Potassium Channel|Potassium Channel]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Human]]
+[[Category: Large Structures]]
 [[Category: Hirsch, J A]]
 [[Category: Strulovich, R]]

5j03

From Proteopedia

Revision as of 06:23, 16 October 2019

Crystal Structure of a chimeric Kv7.2 - Kv7.3 proximal C-terminal Domain in Complex with Calmodulin

Structural highlights

Disease

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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