1orm

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1orm.jpg|left|200px]]
[[Image:1orm.jpg|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1orm |SIZE=350|CAPTION= <scene name='initialview01'>1orm</scene>
+
The line below this paragraph, containing "STRUCTURE_1orm", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND=
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY=
+
or leave the SCENE parameter empty for the default display.
-
|GENE= OMPX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1orm| PDB=1orm | SCENE= }}
-
|RELATEDENTRY=[[1qj8|1QJ8]], [[1qj9|1QJ9]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1orm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1orm OCA], [http://www.ebi.ac.uk/pdbsum/1orm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1orm RCSB]</span>
+
-
}}
+
'''NMR FOLD OF THE OUTER MEMBRANE PROTEIN OMPX IN DHPC MICELLES'''
'''NMR FOLD OF THE OUTER MEMBRANE PROTEIN OMPX IN DHPC MICELLES'''
Line 28: Line 25:
[[Category: Fernandez, C.]]
[[Category: Fernandez, C.]]
[[Category: Wuthrich, K.]]
[[Category: Wuthrich, K.]]
-
[[Category: detergent]]
+
[[Category: Detergent]]
-
[[Category: dhpc]]
+
[[Category: Dhpc]]
-
[[Category: lipid]]
+
[[Category: Lipid]]
-
[[Category: membrane protein]]
+
[[Category: Membrane protein]]
-
[[Category: micelle]]
+
[[Category: Micelle]]
-
[[Category: nmr]]
+
[[Category: Nmr]]
-
[[Category: ompx]]
+
[[Category: Ompx]]
-
[[Category: trosy]]
+
[[Category: Trosy]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:11:51 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:49:21 2008''
+

Revision as of 01:11, 3 May 2008

Image:1orm.jpg

Template:STRUCTURE 1orm

NMR FOLD OF THE OUTER MEMBRANE PROTEIN OMPX IN DHPC MICELLES


Overview

The (2)H,(13)C,(15)N-labeled, 148-residue integral membrane protein OmpX from Escherichia coli was reconstituted with dihexanoyl phosphatidylcholine (DHPC) in mixed micelles of molecular mass of about 60 kDa. Transverse relaxation-optimized spectroscopy (TROSY)-type triple resonance NMR experiments and TROSY-type nuclear Overhauser enhancement spectra were recorded in 2 mM aqueous solutions of these mixed micelles at pH 6.8 and 30 degrees C. Complete sequence-specific NMR assignments for the polypeptide backbone thus have been obtained. The (13)C chemical shifts and the nuclear Overhauser effect data then resulted in the identification of the regular secondary structure elements of OmpX/DHPC in solution and in the collection of an input of conformational constraints for the computation of the global fold of the protein. The same type of polypeptide backbone fold is observed in the presently determined solution structure and the previously reported crystal structure of OmpX determined in the presence of the detergent n-octyltetraoxyethylene. Further structure refinement will have to rely on the additional resonance assignment of partially or fully protonated amino acid side chains, but the present data already demonstrate that relaxation-optimized NMR techniques open novel avenues for studies of structure and function of integral membrane proteins.

About this Structure

1ORM is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Transverse relaxation-optimized NMR spectroscopy with the outer membrane protein OmpX in dihexanoyl phosphatidylcholine micelles., Fernandez C, Adeishvili K, Wuthrich K, Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2358-63. Epub 2001 Feb 20. PMID:11226244 Page seeded by OCA on Sat May 3 04:11:51 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1orm"
Personal tools