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1ose
From Proteopedia
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[[Image:1ose.jpg|left|200px]] | [[Image:1ose.jpg|left|200px]] | ||
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'''PORCINE PANCREATIC ALPHA-AMYLASE COMPLEXED WITH ACARBOSE''' | '''PORCINE PANCREATIC ALPHA-AMYLASE COMPLEXED WITH ACARBOSE''' | ||
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[[Category: Gilles, C.]] | [[Category: Gilles, C.]] | ||
[[Category: Payan, F.]] | [[Category: Payan, F.]] | ||
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| - | [[Category: | + | [[Category: Alpha-amylase]] |
| - | [[Category: | + | [[Category: Hydrolase]] |
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Revision as of 01:13, 3 May 2008
PORCINE PANCREATIC ALPHA-AMYLASE COMPLEXED WITH ACARBOSE
Overview
Two different crystal forms of pig pancreatic alpha-amylase isoenzyme II (PPAII), free and complexed to a carbohydrate inhibitor (acarbose), have been compared together and to previously reported structures of PPAI. A crystal form obtained at 4 degrees C, containing nearly 72% solvent, made it possible to obtain a new complex with acarbose, different from a previous one obtained at 20 degrees C [Qian, M., Buisson, G., Duee, E., Haser, H. & Payan, F. (1994) Biochemistry 33, 6284-6294]. In the present form, six contiguous subsites of the enzyme active site are occupied by the carbohydrate ligand; the structural data indicate that the binding site is capable of holding more than the five glucose units of the scheme proposed through kinetic studies. A monosaccharide ring bridging two protein molecules related by the crystal packing is located on the surface, at a distance of 2.0 nm from the reducing end of the inhibitor ligand; the symmetry-related glucose ring in the crystal lattice is found 1.5 nm away from the non-reducing end of the inhibitor ligand.
About this Structure
1OSE is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.
Reference
Crystal structure of pig pancreatic alpha-amylase isoenzyme II, in complex with the carbohydrate inhibitor acarbose., Gilles C, Astier JP, Marchis-Mouren G, Cambillau C, Payan F, Eur J Biochem. 1996 Jun 1;238(2):561-9. PMID:8681972 Page seeded by OCA on Sat May 3 04:13:41 2008
