1osu

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[[Image:1osu.jpg|left|200px]]
[[Image:1osu.jpg|left|200px]]
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{{Structure
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|PDB= 1osu |SIZE=350|CAPTION= <scene name='initialview01'>1osu</scene>, resolution 1.400&Aring;
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The line below this paragraph, containing "STRUCTURE_1osu", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
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{{STRUCTURE_1osu| PDB=1osu | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1osu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1osu OCA], [http://www.ebi.ac.uk/pdbsum/1osu PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1osu RCSB]</span>
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'''STRUCTURE OF THE RNA HEXAMER, R(UUCGCG), WITH A 5'-UU-OVERHANG EXHIBITING HOOGSTEEN-LIKE TRANS U-U BASE PAIRS'''
'''STRUCTURE OF THE RNA HEXAMER, R(UUCGCG), WITH A 5'-UU-OVERHANG EXHIBITING HOOGSTEEN-LIKE TRANS U-U BASE PAIRS'''
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==About this Structure==
==About this Structure==
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1OSU is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OSU OCA].
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OSU OCA].
==Reference==
==Reference==
The structure of r(UUCGCG) has a 5'-UU-overhang exhibiting Hoogsteen-like trans U.U base pairs., Wahl MC, Rao ST, Sundaralingam M, Nat Struct Biol. 1996 Jan;3(1):24-31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8548450 8548450]
The structure of r(UUCGCG) has a 5'-UU-overhang exhibiting Hoogsteen-like trans U.U base pairs., Wahl MC, Rao ST, Sundaralingam M, Nat Struct Biol. 1996 Jan;3(1):24-31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8548450 8548450]
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[[Category: Protein complex]]
 
[[Category: Rao, S T.]]
[[Category: Rao, S T.]]
[[Category: Sundaralingam, M.]]
[[Category: Sundaralingam, M.]]
[[Category: Wahl, M C.]]
[[Category: Wahl, M C.]]
[[Category: 5'-uu-overhang]]
[[Category: 5'-uu-overhang]]
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[[Category: a-rna]]
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[[Category: A-rna]]
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[[Category: double helix]]
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[[Category: Double helix]]
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[[Category: mismatched]]
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[[Category: Mismatched]]
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[[Category: nicked]]
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[[Category: Nicked]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:14:31 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:49:51 2008''
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Revision as of 01:14, 3 May 2008

Template:STRUCTURE 1osu

STRUCTURE OF THE RNA HEXAMER, R(UUCGCG), WITH A 5'-UU-OVERHANG EXHIBITING HOOGSTEEN-LIKE TRANS U-U BASE PAIRS


Overview

The crystal structure of the RNA fragment, 5'-r(UUCGCG)-3', has been determined at 1.4 A resolution by a combination of single isomorphous replacement and molecular search methods. The 3'-terminal CGCG portion of the hexamer engages in Watson-Crick hydrogen bonding while the 5'-terminal UU-overhang forms novel Hoogsteen-like U.U self-base pairs with the overhang of an adjacent duplex. The U.U pairs display a single conventional hydrogen bond between O4 (U1) and N3 (U8) and a CH-O hydrogen bond between C5-H (U1) and O4 (U8), through the Hoogsteen face of the pyrimidine base U1. This unusual arrangement of one of the bases results in a trans U.U pair on antiparallel strands in contrast to the usual cis base pairs. The structure emphasizes the pronounced polymorphism of U.U pairs and has implications for folding of RNA molecules.

About this Structure

Full crystallographic information is available from OCA.

Reference

The structure of r(UUCGCG) has a 5'-UU-overhang exhibiting Hoogsteen-like trans U.U base pairs., Wahl MC, Rao ST, Sundaralingam M, Nat Struct Biol. 1996 Jan;3(1):24-31. PMID:8548450 Page seeded by OCA on Sat May 3 04:14:31 2008

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