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1osy
From Proteopedia
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'''Crystal structure of FIP-Fve fungal immunomodulatory protein''' | '''Crystal structure of FIP-Fve fungal immunomodulatory protein''' | ||
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[[Category: Seow, S V.]] | [[Category: Seow, S V.]] | ||
[[Category: Shai, V.]] | [[Category: Shai, V.]] | ||
| - | [[Category: | + | [[Category: Fibronectin fold]] |
| - | [[Category: | + | [[Category: Fungal protein]] |
| - | [[Category: | + | [[Category: Hemagglutination]] |
| - | [[Category: | + | [[Category: Immunomodulatory]] |
| - | [[Category: | + | [[Category: Lectin]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:14:39 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 01:14, 3 May 2008
Crystal structure of FIP-Fve fungal immunomodulatory protein
Overview
Fve, a major fruiting body protein from Flammulina velutipes, a mushroom possessing immunomodulatory activity, stimulates lymphocyte mitogenesis, suppresses systemic anaphylaxis reactions and edema, enhances transcription of IL-2, IFN-gamma and TNF-alpha, and hemagglutinates red blood cells. It appears to be a lectin with specificity for complex cell-surface carbohydrates. Fve is a non-covalently linked homodimer containing no Cys, His or Met residues. It shares sequence similarity only to the other fungal immunomodulatory proteins (FIPs) LZ-8, Gts, Vvo and Vvl, all of unknown structure. The 1.7A structure of Fve solved by single anomalous diffraction of NaBr-soaked crystals is novel: each monomer consists of an N-terminal alpha-helix followed by a fibronectin III (FNIII) fold. The FNIII fold is the first instance of "pseudo-h-type" topology, a transition between the seven beta-stranded s-type and the eight beta-stranded h-type topologies. The structure suggests that dimerization, critical for the activity of FIPs, occurs by 3-D domain swapping of the N-terminal helices and is stabilized predominantly by hydrophobic interactions. The structure of Fve is the first in this lectin family to be reported, and the first of an FNIII domain-containing protein of fungal origin.
About this Structure
1OSY is a Single protein structure of sequence from Flammulina velutipes. Full crystallographic information is available from OCA.
Reference
A 1.7A structure of Fve, a member of the new fungal immunomodulatory protein family., Paaventhan P, Joseph JS, Seow SV, Vaday S, Robinson H, Chua KY, Kolatkar PR, J Mol Biol. 2003 Sep 12;332(2):461-70. PMID:12948495 Page seeded by OCA on Sat May 3 04:14:39 2008
