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4xhy

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Revision as of 06:13, 18 April 2018

NADH:FMN oxidoreductase from Paracoccus denitrificans

Structural highlights

4xhy is a 1 chain structure with sequence from Pardp. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	Pden_2689 (PARDP)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

The Pden_2689 gene encoding FerA, an NADH:flavin oxidoreductase required for growth of Paracoccus denitrificans under iron limitation, was cloned and overexpressed as a C-terminally His6-tagged derivative. The binding of substrates and products was detected and quantified by isothermal titration calorimetry and fluorometric titration. FerA binds FMN and FAD with comparable affinity in an enthalpically driven, entropically opposed process. The reduced flavin is bound more loosely than the oxidized one, which was confirmed by a negative shift in the redox potential of FMN after addition of FerA. Initial velocity and substrate analogs inhibition studies showed that FerA follows a random-ordered sequence of substrate (NADH and FMN) binding. The primary kinetic isotope effects from stereospecifically deuterated nicotinamide nucleotides demonstrated that hydride transfer occurs from the pro-S position and contributes to rate limitation for the overall reaction. The crystal structure of FerA revealed a twisted seven-stranded antiparallel beta-barrel similar to that of other short chain flavin reductases. Only minor structural changes around Arg106 took place upon FMN binding. The solution structure FerA derived from small angle X-ray scattering (SAXS) matched the dimer assembly predicted from the crystal structure. Site-directed mutagenesis pinpointed a role of Arg106 and His146 in binding of flavin and NADH, respectively. Pull down experiments performed with cytoplasmic extracts resulted in a negative outcome indicating that FerA might physiologically act without association with other proteins. Rapid kinetics experiments provided evidence for a stabilizing effect of another P. denitrificans protein, the NAD(P)H: acceptor oxidoreducase FerB, against spontaneous oxidation of the FerA-produced dihydroflavin.

Biochemical properties and crystal structure of the flavin reductase FerA from Paracoccus denitrificans.,Sedlacek V, Klumpler T, Marek J, Kucera I Microbiol Res. 2016 Jul-Aug;188-189:9-22. doi: 10.1016/j.micres.2016.04.006. Epub, 2016 Apr 27. PMID:27296958^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sedlacek V, Klumpler T, Marek J, Kucera I. Biochemical properties and crystal structure of the flavin reductase FerA from Paracoccus denitrificans. Microbiol Res. 2016 Jul-Aug;188-189:9-22. doi: 10.1016/j.micres.2016.04.006. Epub, 2016 Apr 27. PMID:27296958 doi:http://dx.doi.org/10.1016/j.micres.2016.04.006

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4xhy"

@@ Line 3: / Line 3: @@
 <StructureSection load='4xhy' size='340' side='right' caption='[[4xhy]], [[Resolution|resolution]] 1.53&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4xhy]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XHY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XHY FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4xhy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pardp Pardp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XHY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XHY FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xhy OCA], [http://pdbe.org/4xhy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xhy RCSB], [http://www.ebi.ac.uk/pdbsum/4xhy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xhy ProSAT]</span></td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Pden_2689 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=318586 PARDP])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xhy OCA], [http://pdbe.org/4xhy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xhy RCSB], [http://www.ebi.ac.uk/pdbsum/4xhy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xhy ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 19: / Line 20: @@
 __TOC__
 </StructureSection>
+[[Category: Pardp]]
 [[Category: Klumpler, T]]
 [[Category: Kucera, I]]

4xhy

From Proteopedia

Revision as of 06:13, 18 April 2018

NADH:FMN oxidoreductase from Paracoccus denitrificans

Structural highlights

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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