5k8e
From Proteopedia
(Difference between revisions)
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5k8e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k8e OCA], [http://pdbe.org/5k8e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k8e RCSB], [http://www.ebi.ac.uk/pdbsum/5k8e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k8e ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5k8e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k8e OCA], [http://pdbe.org/5k8e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k8e RCSB], [http://www.ebi.ac.uk/pdbsum/5k8e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k8e ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | By inspection of the predicted proteome of the fungus Myceliophthora thermophila C1 for VAO-type flavoprotein oxidases, a putative oligosaccharide oxidase was identified. By homologous expression and subsequent purification the respective protein could be obtained. The protein was found to contain a bicovalently bound FAD cofactor. By screening a large number of carbohydrates, several mono- and oligosaccharides could be identified as substrate. The enzyme exhibits a strong substrate preference towards xylooligosaccharides, hence it is named xylooligosaccharide oxidase (XylO). Chemical analyses of the product formed upon oxidation of xylobiose revealed that the oxidation occurs at C1, yielding xylobionate as product. By elucidation of several XylO crystal structures (in complex with a substrate mimic, xylose and xylobiose), the residues that tune the unique substrate specificity and regioselectivity could be identified. The discovery of this novel oligosaccharide oxidase reveals that the VAO-type flavoprotein family harbors oxidases tuned for specific oligosaccharides. The unique substrate profile of XylO hints to a role in the degradation of xylan derived oligosaccharides by the fungus M. thermophila C1. | ||
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| + | Discovery of a xylooligosaccharide oxidase from Myceliophthora thermophila C1.,Ferrari AR, Rozeboom HJ, Dobruchowska JM, van Leeuwen SS, Vugts AS, Koetsier MJ, Visser J, Fraaije MW J Biol Chem. 2016 Sep 14. pii: jbc.M116.741173. PMID:27629413<ref>PMID:27629413</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5k8e" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 09:31, 3 October 2016
Xylooligosaccharide oxidase from Myceliophthora thermophila C1
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Categories: Ferrari, A R | Fraaije, M W | Rozeboom, H J | Cazy | Fad | Oligosaccharide | Oxidoreductase | Xylan | Xylose
