1p22

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[[Image:1p22.gif|left|200px]]
[[Image:1p22.gif|left|200px]]
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{{Structure
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|PDB= 1p22 |SIZE=350|CAPTION= <scene name='initialview01'>1p22</scene>, resolution 2.95&Aring;
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The line below this paragraph, containing "STRUCTURE_1p22", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>
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|GENE= CTNNB1 OR CTNNB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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|DOMAIN=
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{{STRUCTURE_1p22| PDB=1p22 | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p22 OCA], [http://www.ebi.ac.uk/pdbsum/1p22 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1p22 RCSB]</span>
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'''Structure of a beta-TrCP1-Skp1-beta-catenin complex: destruction motif binding and lysine specificity on the SCFbeta-TrCP1 ubiquitin ligase'''
'''Structure of a beta-TrCP1-Skp1-beta-catenin complex: destruction motif binding and lysine specificity on the SCFbeta-TrCP1 ubiquitin ligase'''
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[[Category: Wu, G.]]
[[Category: Wu, G.]]
[[Category: Xu, G.]]
[[Category: Xu, G.]]
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[[Category: degradation]]
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[[Category: Degradation]]
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[[Category: ubiquitination]]
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[[Category: Ubiquitination]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:34:54 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:53:44 2008''
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Revision as of 01:34, 3 May 2008

Image:1p22.gif

Template:STRUCTURE 1p22

Structure of a beta-TrCP1-Skp1-beta-catenin complex: destruction motif binding and lysine specificity on the SCFbeta-TrCP1 ubiquitin ligase


Overview

The SCF ubiquitin ligases catalyze protein ubiquitination in diverse cellular processes. SCFs bind substrates through the interchangeable F box protein subunit, with the >70 human F box proteins allowing the recognition of a wide range of substrates. The F box protein beta-TrCP1 recognizes the doubly phosphorylated DpSGphiXpS destruction motif, present in beta-catenin and IkappaB, and directs the SCF(beta-TrCP1) to ubiquitinate these proteins at specific lysines. The 3.0 A structure of a beta-TrCP1-Skp1-beta-catenin complex reveals the basis of substrate recognition by the beta-TrCP1 WD40 domain. The structure, together with the previous SCF(Skp2) structure, leads to the model of SCF catalyzing ubiquitination by increasing the effective concentration of the substrate lysine at the E2 active site. The model's prediction that the lysine-destruction motif spacing is a determinant of ubiquitination efficiency is confirmed by measuring ubiquitination rates of mutant beta-catenin peptides, solidifying the model and also providing a mechanistic basis for lysine selection.

About this Structure

1P22 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure of a beta-TrCP1-Skp1-beta-catenin complex: destruction motif binding and lysine specificity of the SCF(beta-TrCP1) ubiquitin ligase., Wu G, Xu G, Schulman BA, Jeffrey PD, Harper JW, Pavletich NP, Mol Cell. 2003 Jun;11(6):1445-56. PMID:12820959 Page seeded by OCA on Sat May 3 04:34:54 2008

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