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5gy3
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | The | + | ==The crystal structure of endoglucanase Cel10, a family 8 glycosyl hydrolase from Klebsiella pneumoniae== |
| + | <StructureSection load='5gy3' size='340' side='right' caption='[[5gy3]], [[Resolution|resolution]] 1.77Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5gy3]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GY3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GY3 FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gy3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gy3 OCA], [http://pdbe.org/5gy3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gy3 RCSB], [http://www.ebi.ac.uk/pdbsum/5gy3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gy3 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Cellulases are produced by microorganisms that grow on cellulose biomass. Here, a cellulase, Cel10, was identified in a strain of Klebsiella pneumoniae isolated from Chinese bamboo rat gut. Analysis of substrate specificity showed that Cel10 is able to hydrolyze amorphous carboxymethyl cellulose (CMC) and crystalline forms of cellulose (Avicel and xylan) but is unable to hydrolyze p-nitrophenol beta-D-glucopyranoside (p-NPG), proving that Cel10 is an endoglucanase. A phylogenetic tree analysis indicates that Cel10 belongs to the glycoside hydrolase 8 (GH8) subfamily. In order to further understanding of its substrate specificity, the structure of Cel10 was solved by molecular replacement and refined to 1.76 A resolution. The overall fold is distinct from those of most other enzymes belonging to the GH8 subfamily. Although it forms the typical (alpha/alpha)6-barrel motif fold, like Acetobacterxylinum CMCax, one helix is missing. Structural comparisons with Clostridium thermocellum CelA (CtCelA), the best characterized GH8 endoglucanase, revealed that sugar-recognition subsite -3 is completely missing in Cel10. The absence of this subsite correlates to a more open substrate-binding cleft on the cellooligosaccharide reducing-end side. | ||
| - | + | The crystal structure of the endoglucanase Cel10, a family 8 glycosyl hydrolase from Klebsiella pneumoniae.,Attigani A, Sun L, Wang Q, Liu Y, Bai D, Li S, Huang X Acta Crystallogr F Struct Biol Commun. 2016 Dec 1;72(Pt 12):870-876. Epub 2016, Nov 25. PMID:27917834<ref>PMID:27917834</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| + | <div class="pdbe-citations 5gy3" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Attigani, A]] | [[Category: Attigani, A]] | ||
| - | [[Category: Sun, L | + | [[Category: Li, S P]] |
| - | [[Category: | + | [[Category: Sun, L F]] |
| + | [[Category: Hydrolase]] | ||
Revision as of 21:24, 22 December 2016
The crystal structure of endoglucanase Cel10, a family 8 glycosyl hydrolase from Klebsiella pneumoniae
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