1p4o
From Proteopedia
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'''Structure of Apo unactivated IGF-1R KInase domain at 1.5A resolution.''' | '''Structure of Apo unactivated IGF-1R KInase domain at 1.5A resolution.''' | ||
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[[Category: Munshi, S.]] | [[Category: Munshi, S.]] | ||
[[Category: Waxman, L.]] | [[Category: Waxman, L.]] | ||
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Revision as of 01:40, 3 May 2008
Structure of Apo unactivated IGF-1R KInase domain at 1.5A resolution.
Contents |
Overview
The crystal structure of the wild-type unactivated kinase domain (IGFRK-0P) of insulin-like growth factor-1 receptor has been reported previously at 2.7 A resolution [Munshi et al. (2002), J. Biol. Chem. 277, 38797-38802]. In order to obtain a high-resolution structure, a number of variants of IGFRK-0P were prepared and screened for crystallization. A double mutant with E1067A and E1069A substitutions within the kinase-insert region resulted in crystals that diffracted to 1.5 A resolution. Overall, the structure of the mutant IGFRK-0P is similar to that of the wild-type IGFRK-0P structure, with the exception of the previously disordered kinase-insert region in the wild type having become fixed. In addition, amino-acid residues 947-952 at the N-terminus are well defined in the mutant structure. The monomeric protein structure is folded into two lobes connected by a hinge region, with the catalytic center situated at the interface of the two lobes. Two molecules of IGFRK-0P in the asymmetric unit are associated as a dimer and two different types of dimers with their ATP-binding clefts either facing towards or away from each other are observed. The current refined model consists of a dimer and 635 water molecules.
Disease
Known disease associated with this structure: Intrauterine and postnatal growth retardation OMIM:[147370]
About this Structure
1P4O is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of apo, unactivated insulin-like growth factor-1 receptor kinase at 1.5 A resolution., Munshi S, Hall DL, Kornienko M, Darke PL, Kuo LC, Acta Crystallogr D Biol Crystallogr. 2003 Oct;59(Pt 10):1725-30. Epub 2003, Sep 19. PMID:14501110 Page seeded by OCA on Sat May 3 04:40:55 2008
