1p51
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1p51.gif|left|200px]] | [[Image:1p51.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1p51", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_1p51| PDB=1p51 | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Anabaena HU-DNA cocrystal structure (AHU6)''' | '''Anabaena HU-DNA cocrystal structure (AHU6)''' | ||
| Line 29: | Line 26: | ||
[[Category: Swinger, K K.]] | [[Category: Swinger, K K.]] | ||
[[Category: Zhang, Y.]] | [[Category: Zhang, Y.]] | ||
| - | [[Category: | + | [[Category: Dna bending]] |
| - | [[Category: | + | [[Category: Hu]] |
| - | [[Category: | + | [[Category: Protein-dna complex]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:41:48 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 01:41, 3 May 2008
Anabaena HU-DNA cocrystal structure (AHU6)
Overview
HU and IHF are members of a family of prokaryotic proteins that interact with the DNA minor groove in a sequence-specific (IHF) or non-specific (HU) manner to induce and/or stabilize DNA bending. HU plays architectural roles in replication initiation, transcription regulation and site-specific recombination, and is associated with bacterial nucleoids. Cocrystal structures of Anabaena HU bound to DNA (1P71, 1P78, 1P51) reveal that while underlying proline intercalation and asymmetric charge neutralization mechanisms of DNA bending are similar for IHF and HU, HU stabilizes different DNA bend angles ( approximately 105-140 degrees ). The two bend angles within a single HU complex are not coplanar, and the resulting dihedral angle is consistent with negative supercoiling. Comparison of HU-DNA and IHF-DNA structures suggests that sharper bending is correlated with longer DNA binding sites and smaller dihedral angles. An HU-induced bend may be better modeled as a hinge, not a rigid bend. The ability to induce or stabilize varying bend angles is consistent with HU's role as an architectural cofactor in many different systems that may require differing geometries.
About this Structure
1P51 is a Single protein structure of sequence from Anabaena sp.. Full crystallographic information is available from OCA.
Reference
Flexible DNA bending in HU-DNA cocrystal structures., Swinger KK, Lemberg KM, Zhang Y, Rice PA, EMBO J. 2003 Jul 15;22(14):3749-60. PMID:12853489 Page seeded by OCA on Sat May 3 04:41:48 2008
