1p84

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[[Image:1p84.gif|left|200px]]
[[Image:1p84.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1p84 |SIZE=350|CAPTION= <scene name='initialview01'>1p84</scene>, resolution 2.50&Aring;
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The line below this paragraph, containing "STRUCTURE_1p84", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=3PE:1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE'>3PE</scene>, <scene name='pdbligand=3PH:1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE'>3PH</scene>, <scene name='pdbligand=CDL:CARDIOLIPIN'>CDL</scene>, <scene name='pdbligand=DBT:5-HEPTYL-6-HYDROXY-1,3-BENZOTHIAZOLE-4,7-DIONE'>DBT</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PC1:1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE'>PC1</scene>, <scene name='pdbligand=UMQ:UNDECYL-MALTOSIDE'>UMQ</scene>, <scene name='pdbligand=UQ6:5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL'>UQ6</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquinol--cytochrome-c_reductase Ubiquinol--cytochrome-c reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.2.2 1.10.2.2] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1p84| PDB=1p84 | SCENE= }}
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|RELATEDENTRY=[[1kb9|1kb9]], [[1ezv|1ezv]], [[1kyo|1kyo]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p84 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p84 OCA], [http://www.ebi.ac.uk/pdbsum/1p84 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1p84 RCSB]</span>
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}}
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'''HDBT inhibited Yeast Cytochrome bc1 Complex'''
'''HDBT inhibited Yeast Cytochrome bc1 Complex'''
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[[Category: Palsdottir, H.]]
[[Category: Palsdottir, H.]]
[[Category: Trumpower, B L.]]
[[Category: Trumpower, B L.]]
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[[Category: complex iii]]
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[[Category: Complex iii]]
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[[Category: cytochrome bc1 complex]]
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[[Category: Cytochrome bc1 complex]]
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[[Category: cytochrome c oxidoreductase]]
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[[Category: Cytochrome c oxidoreductase]]
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[[Category: hhdbt]]
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[[Category: Hhdbt]]
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[[Category: hydroxyquinone]]
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[[Category: Hydroxyquinone]]
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[[Category: membrane protein]]
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[[Category: Membrane protein]]
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[[Category: phospholipid]]
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[[Category: Phospholipid]]
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[[Category: qo site]]
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[[Category: Qo site]]
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[[Category: ubiquinol]]
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[[Category: Ubiquinol]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:48:07 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:56:15 2008''
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Revision as of 01:48, 3 May 2008

Image:1p84.gif

Template:STRUCTURE 1p84

HDBT inhibited Yeast Cytochrome bc1 Complex


Overview

Bifurcated electron transfer during ubiquinol oxidation is the key reaction of cytochrome bc1 complex catalysis. Binding of the competitive inhibitor 5-n-heptyl-6-hydroxy-4,7-dioxobenzothiazole to the Qo site of the cytochrome bc1 complex from Saccharomyces cerevisiae was analyzed by x-ray crystallography. This alkylhydroxydioxobenzothiazole is bound in its ionized form as evident from the crystal structure and confirmed by spectroscopic analysis, consistent with a measured pKa = 6.1 of the hydroxy group in detergent micelles. Stabilizing forces for the hydroxyquinone anion inhibitor include a polarized hydrogen bond to the iron-sulfur cluster ligand His181 and on-edge interactions via weak hydrogen bonds with cytochrome b residue Tyr279. The hydroxy group of the latter contributes to stabilization of the Rieske protein in the b-position by donating a hydrogen bond. The reported pH dependence of inhibition with lower efficacy at alkaline pH is attributed to the protonation state of His181 with a pKa of 7.5. Glu272, a proposed primary ligand and proton acceptor of ubiquinol, is not bound to the carbonyl group of the hydroxydioxobenzothiazole ring but is rotated out of the binding pocket toward the heme bL propionate A, to which it is hydrogen-bonded via a single water molecule. The observed hydrogen bonding pattern provides experimental evidence for the previously proposed proton exit pathway involving the heme propionate and a chain of water molecules. Binding of the alkyl-6-hydroxy-4,7-dioxobenzothiazole is discussed as resembling an intermediate step of ubiquinol oxidation, supporting a single occupancy model at the Qo site.

About this Structure

1P84 is a Protein complex structure of sequences from Mus musculus and Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Structure of the yeast cytochrome bc1 complex with a hydroxyquinone anion Qo site inhibitor bound., Palsdottir H, Lojero CG, Trumpower BL, Hunte C, J Biol Chem. 2003 Aug 15;278(33):31303-11. Epub 2003 Jun 2. PMID:12782631 Page seeded by OCA on Sat May 3 04:48:07 2008

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