1p8q
From Proteopedia
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[[Image:1p8q.gif|left|200px]] | [[Image:1p8q.gif|left|200px]] | ||
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'''Structural and Functional Importance of First-Shell Metal Ligands in the Binuclear Cluster of Arginase I.''' | '''Structural and Functional Importance of First-Shell Metal Ligands in the Binuclear Cluster of Arginase I.''' | ||
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[[Category: Christianson, D W.]] | [[Category: Christianson, D W.]] | ||
[[Category: Emig, F A.]] | [[Category: Emig, F A.]] | ||
| - | [[Category: | + | [[Category: Arginine metabolism]] |
| - | [[Category: | + | [[Category: Binuclear manganese cluster]] |
| - | [[Category: | + | [[Category: Hydrolase]] |
| - | [[Category: | + | [[Category: Urea cycle]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:49:38 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 01:49, 3 May 2008
Structural and Functional Importance of First-Shell Metal Ligands in the Binuclear Cluster of Arginase I.
Overview
Arginase is a binuclear manganese metalloenzyme that hydrolyzes l-arginine to form l-ornithine and urea. The three-dimensional structures of D128E, D128N, D232A, D232C, D234E, H101N, and H101E arginases I have been determined by X-ray crystallographic methods to elucidate the roles of the first-shell metal ligands in the stability and catalytic activity of the enzyme. This work represents the first structure-based dissection of the binuclear manganese cluster using site-directed mutagenesis and X-ray crystallography. Substitution of the metal ligands compromises the catalytic activity of the enzyme, either by the loss or disruption of the metal cluster or the nucleophilic metal-bridging hydroxide ion. However, the substitution of the metal ligands or the reduction of Mn(2+)(A) or Mn(2+)(B) occupancy does not compromise enzyme-substrate affinity as reflected by K(M), which remains relatively invariant across this series of arginase variants. This implicates a nonmetal binding site for substrate l-arginine in the precatalytic Michaelis complex, as proposed based on analysis of the native enzyme structure (Kanyo, Z. F., Scolnick, L. R., Ash, D. E., and Christianson, D. W. (1996) Nature 383, 554-557).
About this Structure
1P8Q is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Structural and functional importance of first-shell metal ligands in the binuclear manganese cluster of arginase I., Cama E, Emig FA, Ash DE, Christianson DW, Biochemistry. 2003 Jul 1;42(25):7748-58. PMID:12820884 Page seeded by OCA on Sat May 3 04:49:38 2008
