1p91

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[[Image:1p91.gif|left|200px]]
[[Image:1p91.gif|left|200px]]
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{{Structure
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|PDB= 1p91 |SIZE=350|CAPTION= <scene name='initialview01'>1p91</scene>, resolution 2.80&Aring;
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The line below this paragraph, containing "STRUCTURE_1p91", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_(guanine-N(1)-)-methyltransferase rRNA (guanine-N(1)-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.51 2.1.1.51] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= RRMA OR B1822 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=PRK11088 rrmA], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam08241 Methyltransf_11]</span>
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{{STRUCTURE_1p91| PDB=1p91 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p91 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p91 OCA], [http://www.ebi.ac.uk/pdbsum/1p91 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1p91 RCSB]</span>
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}}
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'''Crystal Structure of RlmA(I) enzyme: 23S rRNA n1-G745 methyltransferase (NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET ER19)'''
'''Crystal Structure of RlmA(I) enzyme: 23S rRNA n1-G745 methyltransferase (NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET ER19)'''
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: rRNA (guanine-N(1)-)-methyltransferase]]
 
[[Category: Acton, T.]]
[[Category: Acton, T.]]
[[Category: Arnold, E.]]
[[Category: Arnold, E.]]
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[[Category: NESG, Northeast Structural Genomics Consortium.]]
[[Category: NESG, Northeast Structural Genomics Consortium.]]
[[Category: 23s rrna]]
[[Category: 23s rrna]]
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[[Category: er19]]
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[[Category: Er19]]
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[[Category: g745]]
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[[Category: G745]]
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[[Category: methyltransferase]]
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[[Category: Methyltransferase]]
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[[Category: nesg]]
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[[Category: Nesg]]
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[[Category: northeast structural genomics consortium]]
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[[Category: Northeast structural genomics consortium]]
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[[Category: protein structure initiative]]
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[[Category: Protein structure initiative]]
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[[Category: psi]]
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[[Category: Psi]]
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[[Category: rlma]]
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[[Category: Rlma]]
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[[Category: rrma]]
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[[Category: Rrma]]
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[[Category: structural genomic]]
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[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:50:07 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:56:35 2008''
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Revision as of 01:50, 3 May 2008

Image:1p91.gif

Template:STRUCTURE 1p91

Crystal Structure of RlmA(I) enzyme: 23S rRNA n1-G745 methyltransferase (NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET ER19)


Overview

The RlmA class of enzymes (RlmA(I) and RlmA(II)) catalyzes N1-methylation of a guanine base (G745 in Gram-negative and G748 in Gram-positive bacteria) of hairpin 35 of 23S rRNA. We have determined the crystal structure of Escherichia coli RlmA(I) at 2.8-A resolution, providing 3D structure information for the RlmA class of RNA methyltransferases. The dimeric protein structure exhibits features that provide new insights into its molecular function. Each RlmA(I) molecule has a Zn-binding domain, responsible for specific recognition and binding of its rRNA substrate, and a methyltransferase domain. The asymmetric RlmA(I) dimer observed in the crystal structure has a well defined W-shaped RNA-binding cleft. Two S-adenosyl-l-methionine substrate molecules are located at the two valleys of the W-shaped RNA-binding cleft. The unique shape of the RNA-binding cleft, different from that of known RNA-binding proteins, is highly specific and structurally complements the 3D structure of hairpin 35 of bacterial 23S rRNA. Apart from the hairpin 35, parts of hairpins 33 and 34 also interact with the RlmA(I) dimer.

About this Structure

1P91 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of RlmAI: implications for understanding the 23S rRNA G745/G748-methylation at the macrolide antibiotic-binding site., Das K, Acton T, Chiang Y, Shih L, Arnold E, Montelione GT, Proc Natl Acad Sci U S A. 2004 Mar 23;101(12):4041-6. Epub 2004 Mar 3. PMID:14999102 Page seeded by OCA on Sat May 3 04:50:07 2008

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