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1p9f
From Proteopedia
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[[Image:1p9f.jpg|left|200px]] | [[Image:1p9f.jpg|left|200px]] | ||
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'''NMR Structure of Neurokinin B from DYANA''' | '''NMR Structure of Neurokinin B from DYANA''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1P9F is a [[Single protein]] structure | + | 1P9F is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9F OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Cowsik, S M.]] | [[Category: Cowsik, S M.]] | ||
[[Category: Mantha, A K.]] | [[Category: Mantha, A K.]] | ||
| - | [[Category: | + | [[Category: Neuropeptide]] |
| - | [[Category: | + | [[Category: Protein]] |
| - | [[Category: | + | [[Category: Structures from dyana]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:50:59 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 01:50, 3 May 2008
NMR Structure of Neurokinin B from DYANA
Overview
Neurokinin B (NKB), a decapeptide of mammalian origin exhibits a variety of biological activities such as regulatory functions in reproduction, pre-eclampsia and neuroprotection in Alzheimer's disease. In order to gain insight into structure-function relationship, three-dimensional structure of NKB has been investigated using CD spectropolarimetry and two-dimensional proton nuclear magnetic resonance (2D 1H-NMR) spectroscopy in aqueous and membrane mimetic solvents. Unambiguous NMR assignments of resonances have been made with the aid of correlation spectroscopy (DQF-COSY and TOCSY) experiments and Nuclear Overhauser Effect Spectroscopy (NOESY) experiments. Distance constraints obtained from the NMR data have been used to generate a family of structures, which have been refined using restrained energy minimization and dynamics. Our data show that a helical structure is induced in NKB, in presence of perdeuterated dodecyl phosphocholine (DPC) micelles, a membrane model system. Further, the conformation adopted by NKB in presence of DPC micelles represents a structural motif typical of neurokinin-3 selective agonists.
About this Structure
1P9F is a Single protein structure. Full crystallographic information is available from OCA.
Reference
Three dimensional structure of mammalian tachykinin peptide neurokinin B bound to lipid micelles., Mantha AK, Chandrashekar IR, Baquer NZ, Cowsik SM, J Biomol Struct Dyn. 2004 Oct;22(2):137-48. PMID:15317475 Page seeded by OCA on Sat May 3 04:50:59 2008
