1p9p
From Proteopedia
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'''The Crystal Structure of a M1G37 tRNA Methyltransferase, TrmD''' | '''The Crystal Structure of a M1G37 tRNA Methyltransferase, TrmD''' | ||
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[[Category: Escherichia coli, escherichia coli o6, escherichia coli o157:h7, and shigella flexneri]] | [[Category: Escherichia coli, escherichia coli o6, escherichia coli o157:h7, and shigella flexneri]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: tRNA (guanine-N(1)-)-methyltransferase]] | ||
[[Category: Andraos-Selim, C.]] | [[Category: Andraos-Selim, C.]] | ||
[[Category: Elkins, P A.]] | [[Category: Elkins, P A.]] | ||
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[[Category: Watts, J M.]] | [[Category: Watts, J M.]] | ||
[[Category: Zalacain, M.]] | [[Category: Zalacain, M.]] | ||
| - | [[Category: | + | [[Category: Adomet]] |
| - | [[Category: | + | [[Category: Knot]] |
| - | [[Category: | + | [[Category: Methyltransferase]] |
| - | [[Category: | + | [[Category: S-adenosylmethionine]] |
| - | [[Category: | + | [[Category: Spou]] |
| - | [[Category: | + | [[Category: Spout]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:51:37 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 01:51, 3 May 2008
The Crystal Structure of a M1G37 tRNA Methyltransferase, TrmD
Overview
The crystal structure of Escherichia coli tRNA (guanosine-1) methyltransferase (TrmD) complexed with S-adenosyl homocysteine (AdoHcy) has been determined at 2.5A resolution. TrmD, which methylates G37 of tRNAs containing the sequence G36pG37, is a homo-dimer. Each monomer consists of a C-terminal domain connected by a flexible linker to an N-terminal AdoMet-binding domain. The two bound AdoHcy moieties are buried at the bottom of deep clefts. The dimer structure appears integral to the formation of the catalytic center of the enzyme and this arrangement strongly suggests that the anticodon loop of tRNA fits into one of these clefts for methyl transfer to occur. In addition, adjacent hydrophobic sites in the cleft delineate a defined pocket, which may accommodate the GpG sequence during catalysis. The dimer contains two deep trefoil peptide knots and a peptide loop extending from each knot embraces the AdoHcy adenine ring. Mutational analyses demonstrate that the knot is important for AdoMet binding and catalytic activity, and that the C-terminal domain is not only required for tRNA binding but plays a functional role in catalytic activity.
About this Structure
1P9P is a Single protein structure of sequence from Escherichia coli, escherichia coli o6, escherichia coli o157:h7, and shigella flexneri. Full crystallographic information is available from OCA.
Reference
Insights into catalysis by a knotted TrmD tRNA methyltransferase., Elkins PA, Watts JM, Zalacain M, van Thiel A, Vitazka PR, Redlak M, Andraos-Selim C, Rastinejad F, Holmes WM, J Mol Biol. 2003 Nov 7;333(5):931-49. PMID:14583191 Page seeded by OCA on Sat May 3 04:51:37 2008
Categories: Escherichia coli, escherichia coli o6, escherichia coli o157:h7, and shigella flexneri | Single protein | Andraos-Selim, C. | Elkins, P A. | Holmes, W M. | Rastinejad, F. | Redlak, M. | Thiel, A Van. | Vitaszka, P R. | Watts, J M. | Zalacain, M. | Adomet | Knot | Methyltransferase | S-adenosylmethionine | Spou | Spout
