1pae

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[[Image:1pae.gif|left|200px]]
[[Image:1pae.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1pae |SIZE=350|CAPTION= <scene name='initialview01'>1pae</scene>, resolution 2.70&Aring;
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The line below this paragraph, containing "STRUCTURE_1pae", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CSE:SELENOCYSTEINE'>CSE</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SE:SELENIUM+ATOM'>SE</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Nucleoside-diphosphate_kinase Nucleoside-diphosphate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.6 2.7.4.6] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= ndk ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 Dictyostelium discoideum])
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-->
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|DOMAIN=
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{{STRUCTURE_1pae| PDB=1pae | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pae FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pae OCA], [http://www.ebi.ac.uk/pdbsum/1pae PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1pae RCSB]</span>
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}}
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'''nucleoside diphosphate kinase'''
'''nucleoside diphosphate kinase'''
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[[Category: Strub, J M.]]
[[Category: Strub, J M.]]
[[Category: Strub, M P.]]
[[Category: Strub, M P.]]
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[[Category: selenocysteine selenomethionine]]
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[[Category: Selenocysteine selenomethionine]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:52:50 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:57:06 2008''
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Revision as of 01:52, 3 May 2008

Image:1pae.gif

Template:STRUCTURE 1pae

nucleoside diphosphate kinase


Overview

A protocol for the quantitative incorporation of both selenomethionine and selenocysteine into recombinant proteins overexpressed in Escherichia coli is described. This methodology is based on the use of a suitable cysteine auxotrophic strain and a minimal medium supplemented with selenium-labeled methionine and cysteine. The proteins chosen for these studies are the cathelin-like motif of protegrin-3 and a nucleoside-diphosphate kinase. Analysis of the purified proteins by electrospray mass spectrometry and X-ray crystallography revealed that both cysteine and methionine residues were isomorphously replaced by selenocysteine and selenomethionine. Moreover, selenocysteines allowed the formation of unstrained and stable diselenide bridges in place of the canonical disulfide bonds. In addition, we showed that NDP kinase contains a selenocysteine adduct on Cys122. This novel selenium double-labeling method is proposed as a general approach to increase the efficiency of the MAD technique used for phase determination in protein crystallography.

About this Structure

1PAE is a Single protein structure of sequence from Dictyostelium discoideum. Full crystallographic information is available from OCA.

Reference

Selenomethionine and selenocysteine double labeling strategy for crystallographic phasing., Strub MP, Hoh F, Sanchez JF, Strub JM, Bock A, Aumelas A, Dumas C, Structure. 2003 Nov;11(11):1359-67. PMID:14604526 Page seeded by OCA on Sat May 3 04:52:50 2008

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