User:Wally Novak/Sandbox Brown
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== Introduction == | == Introduction == | ||
<StructureSection load='4qyz' size='340' side='right' caption='CRISPR RNA-guided surveillance complex with target ssDNA' scene=''> | <StructureSection load='4qyz' size='340' side='right' caption='CRISPR RNA-guided surveillance complex with target ssDNA' scene=''> | ||
| - | Cas9 is a large multifunctional protein that plays a central role in the CRISPR-Cas adaptive defense mechanism found in a vast amount of bacteria and archaea <ref>DOI 10.1126/science.1258096</ref>. | + | Cas9 is a large multifunctional protein that plays a central role in the CRISPR-Cas adaptive defense mechanism found in a vast amount of bacteria and archaea <ref>DOI 10.1126/science.1258096</ref>. It accomplishes this through the use of antisense RNAs which serve as signatures from past viral invasions <ref>DOI 10.1186/1745-6150-1-7</ref>. The adaptive immunity occurs in three stages: insertion of invading DNA into CRISPR locus, transcription of precursor crRNA from CRISPR locus that will be used to generate crRNA, and crRNA-directed cleavage of foreign nucleic acids by cas9 <ref>DOI 10.1126/science.1258096</ref>. |
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Revision as of 23:19, 10 October 2016
Introduction
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References
- ↑ Doudna JA, Charpentier E. Genome editing. The new frontier of genome engineering with CRISPR-Cas9. Science. 2014 Nov 28;346(6213):1258096. doi: 10.1126/science.1258096. PMID:25430774 doi:http://dx.doi.org/10.1126/science.1258096
- ↑ Makarova KS, Grishin NV, Shabalina SA, Wolf YI, Koonin EV. A putative RNA-interference-based immune system in prokaryotes: computational analysis of the predicted enzymatic machinery, functional analogies with eukaryotic RNAi, and hypothetical mechanisms of action. Biol Direct. 2006 Mar 16;1:7. PMID:16545108 doi:http://dx.doi.org/10.1186/1745-6150-1-7
- ↑ Doudna JA, Charpentier E. Genome editing. The new frontier of genome engineering with CRISPR-Cas9. Science. 2014 Nov 28;346(6213):1258096. doi: 10.1126/science.1258096. PMID:25430774 doi:http://dx.doi.org/10.1126/science.1258096
- ↑ Hanson, R. M., Prilusky, J., Renjian, Z., Nakane, T. and Sussman, J. L. (2013), JSmol and the Next-Generation Web-Based Representation of 3D Molecular Structure as Applied to Proteopedia. Isr. J. Chem., 53:207-216. doi:http://dx.doi.org/10.1002/ijch.201300024
- ↑ Herraez A. Biomolecules in the computer: Jmol to the rescue. Biochem Mol Biol Educ. 2006 Jul;34(4):255-61. doi: 10.1002/bmb.2006.494034042644. PMID:21638687 doi:10.1002/bmb.2006.494034042644
