User:Wally Novak/Sandbox Brown
From Proteopedia
(Difference between revisions)
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== Overall Structure == | == Overall Structure == | ||
| - | The cas9 protein complex has a seahorse shaped structure that is composed of 11 cas subunits. Cascade (CRISPR-associated complex for antiviral defense) is from the type-I CRISPR-cas system, and the crystal structure of this surveillance complex gives insight into the overall structure. ('''IMAGE''') The body is comprised of six subunits (Cas7.1-7.6) wrapped around the crRNA in a helical filament with a dimer of Cse2 in the center <ref name='four'>DOI 10.1126/science.1256996</ref>. The head of the Cas7 body is capped by Cas6e and the 3' end of the crRNA while the 5' end and Cas5 cap the tail. | + | The cas9 protein complex has a seahorse shaped structure that is composed of 11 cas subunits. Cascade (CRISPR-associated complex for antiviral defense) is from the type-I CRISPR-cas system, and the crystal structure of this surveillance complex gives insight into the overall structure. ('''IMAGE''') The body is comprised of six subunits (Cas7.1-7.6) wrapped around the crRNA in a helical filament with a dimer of Cse2 in the center <ref name='four'>DOI 10.1126/science.1256996</ref>. The head of the Cas7 body is capped by Cas6e and the 3' end of the crRNA while the 5' end and Cas5 cap the tail. The N-terminal end of Cse1 is also at the tail and the C-terminal end contains a bundle of 4 helices that contact Cse2.2. The Cse2 dimer, Cas7 filament, and four-helix bundle of Cse1 form a groove immediately next to guide region of the crRNA. This is where the ssDNA target fits into the complex <ref name='four'/>. |
== Active Site Structure == | == Active Site Structure == | ||
| - | == Structural highlights == | ||
| - | This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes. | ||
</StructureSection> | </StructureSection> | ||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 01:17, 11 October 2016
Introduction
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References
- ↑ 1.0 1.1 1.2 Doudna JA, Charpentier E. Genome editing. The new frontier of genome engineering with CRISPR-Cas9. Science. 2014 Nov 28;346(6213):1258096. doi: 10.1126/science.1258096. PMID:25430774 doi:http://dx.doi.org/10.1126/science.1258096
- ↑ Makarova KS, Grishin NV, Shabalina SA, Wolf YI, Koonin EV. A putative RNA-interference-based immune system in prokaryotes: computational analysis of the predicted enzymatic machinery, functional analogies with eukaryotic RNAi, and hypothetical mechanisms of action. Biol Direct. 2006 Mar 16;1:7. PMID:16545108 doi:http://dx.doi.org/10.1186/1745-6150-1-7
- ↑ Deltcheva E, Chylinski K, Sharma CM, Gonzales K, Chao Y, Pirzada ZA, Eckert MR, Vogel J, Charpentier E. CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III. Nature. 2011 Mar 31;471(7340):602-7. doi: 10.1038/nature09886. PMID:21455174 doi:http://dx.doi.org/10.1038/nature09886
- ↑ 4.0 4.1 Mulepati S, Heroux A, Bailey S. Crystal structure of a CRISPR RNA-guided surveillance complex bound to a ssDNA target. Science. 2014 Aug 14. pii: 1256996. PMID:25123481 doi:http://dx.doi.org/10.1126/science.1256996
