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1pc9
From Proteopedia
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[[Image:1pc9.gif|left|200px]] | [[Image:1pc9.gif|left|200px]] | ||
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'''Crystal Structure of BnSP-6, a Lys49-Phospholipase A2''' | '''Crystal Structure of BnSP-6, a Lys49-Phospholipase A2''' | ||
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Crystal structures of BnSP-7 and BnSP-6, two Lys49-phospholipases A(2): quaternary structure and inhibition mechanism insights., Magro AJ, Soares AM, Giglio JR, Fontes MR, Biochem Biophys Res Commun. 2003 Nov 21;311(3):713-20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14623331 14623331] | Crystal structures of BnSP-7 and BnSP-6, two Lys49-phospholipases A(2): quaternary structure and inhibition mechanism insights., Magro AJ, Soares AM, Giglio JR, Fontes MR, Biochem Biophys Res Commun. 2003 Nov 21;311(3):713-20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14623331 14623331] | ||
[[Category: Bothrops neuwiedi pauloensis]] | [[Category: Bothrops neuwiedi pauloensis]] | ||
| - | [[Category: Phospholipase A(2)]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Fontes, M R.M.]] | [[Category: Fontes, M R.M.]] | ||
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[[Category: Magro, A J.]] | [[Category: Magro, A J.]] | ||
[[Category: Soares, A M.]] | [[Category: Soares, A M.]] | ||
| - | [[Category: | + | [[Category: Bothrop]] |
| - | [[Category: | + | [[Category: Crystal structure]] |
| - | [[Category: | + | [[Category: Lys49-phospholipase a2]] |
| - | [[Category: | + | [[Category: Myotoxin]] |
| - | [[Category: | + | [[Category: Venom.]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:55:53 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 01:55, 3 May 2008
Crystal Structure of BnSP-6, a Lys49-Phospholipase A2
Overview
Phospholipases A(2) are components of Bothrops venoms responsible for disruption of cell membrane integrity via hydrolysis of its phospholipids. A class of PLA(2)-like proteins has been described which despite PLA(2) activity on artificial substrate, due to a D49K mutation, is still highly myonecrotic. This work reports the X-ray structure determination of two Lys49-PLA(2)s from Bothrops neuwiedi pauloensis (BnSP-7 and BnSP-6) and, for the first time, the comparison of eight dimeric Lys49-PLA(2)s. This comparison reveals that there are not just two ("open" and "closed") but at least six different conformations. The binding of fatty acid observed in three recent Lys49-PLA(2) structures seems to be independent of their quaternary conformation. Cys29 polarization by Lys122 is not significant for BnSP-7 and BnSP-6 or other structures not bound by fatty acids. These structures may be in an "active" state when nothing is bound to them and the Lys122/Cys29 interactions are weak or absent.
About this Structure
1PC9 is a Single protein structure of sequence from Bothrops neuwiedi pauloensis. Full crystallographic information is available from OCA.
Reference
Crystal structures of BnSP-7 and BnSP-6, two Lys49-phospholipases A(2): quaternary structure and inhibition mechanism insights., Magro AJ, Soares AM, Giglio JR, Fontes MR, Biochem Biophys Res Commun. 2003 Nov 21;311(3):713-20. PMID:14623331 Page seeded by OCA on Sat May 3 04:55:53 2008
