1pfi
From Proteopedia
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'''PF1 VIRUS STRUCTURE: HELICAL COAT PROTEIN AND DNA WITH PARAXIAL PHOSPHATES''' | '''PF1 VIRUS STRUCTURE: HELICAL COAT PROTEIN AND DNA WITH PARAXIAL PHOSPHATES''' | ||
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[[Category: Day, L A.]] | [[Category: Day, L A.]] | ||
[[Category: Liu, D J.]] | [[Category: Liu, D J.]] | ||
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Revision as of 02:01, 3 May 2008
PF1 VIRUS STRUCTURE: HELICAL COAT PROTEIN AND DNA WITH PARAXIAL PHOSPHATES
Overview
The helical path of the DNA in filamentous bacteriophage Pf1 was deduced from different kinds of existing structural information, including results from x-ray fiber diffraction. The DNA has the same pitch, 16 angstroms, as the surrounding helix of protein subunits; the rise and rotation per nucleotides are 6.1 angstroms and 132 degrees, respectively; and the phosphates are 2.5 angstroms from the axis. The DNA in Pf1 is, therefore, the most extended and twisted DNA structure known. On the basis of the DNA structure and extensive additional information about the protein, a model of the virion is proposed. In the model, the DNA bases reach out, into the protein, and the lysine and arginine side chains reach in, between the DNA bases, to stabilize the paraxial phosphate charges; the conformation of the protein subunit is a combination of alpha and 3(10) helices.
About this Structure
1PFI is a Single protein structure of sequence from Pseudomonas phage pf1. Full crystallographic information is available from OCA.
Reference
Pf1 virus structure: helical coat protein and DNA with paraxial phosphates., Liu DJ, Day LA, Science. 1994 Jul 29;265(5172):671-4. PMID:8036516 Page seeded by OCA on Sat May 3 05:01:35 2008