5l71

From Proteopedia

(Difference between revisions)

Revision as of 06:59, 5 August 2020

Crystal structure of mouse phospholipid hydroperoxide glutathione peroxidase 4 (GPx4)

Structural highlights

5l71 is a 1 chain structure with sequence from Lk3 transgenic mice. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	Gpx4 (LK3 transgenic mice)
Activity:	Phospholipid-hydroperoxide glutathione peroxidase, with EC number 1.11.1.12
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[GPX41_MOUSE] Protects cells against membrane lipid peroxidation and cell death (PubMed:12566075). Required for normal sperm development and male fertility (PubMed:19417079). Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development (PubMed:12566075). Protects from radiation and oxidative damage (PubMed:12566075). Essential for maturation and survival of photoreceptor cells (PubMed:22207760). Plays a role in a primary T cell response to viral and parasitic infection by protecting T cells from ferroptosis, a cell death resulting from an iron-dependent accumulation of lipid reactive oxygen species, and by supporting T cell expansion (PubMed:25824823).^[1] ^[2] ^[3] ^[4] ^[5]

Publication Abstract from PubMed

The mammalian glutathione peroxidase (GPx) family is a key component of the cellular antioxidative defence system. Within this family, GPx4 has unique features as it accepts a large class of hydroperoxy lipid substrates and has a plethora of biological functions, including sperm maturation, regulation of apoptosis and cerebral embryogenesis. In this paper, the structure of the cytoplasmic isoform of mouse phospholipid hydroperoxide glutathione peroxidase (O70325-2 GPx4) with selenocysteine 46 mutated to cysteine is reported solved at 1.8 A resolution using X-ray crystallography. Furthermore, solution data of an isotope-labelled GPx protein are presented.

Crystal and solution structural studies of mouse phospholipid hydroperoxide glutathione peroxidase 4.,Janowski R, Scanu S, Niessing D, Madl T Acta Crystallogr F Struct Biol Commun. 2016 Oct 1;72(Pt 10):743-749. Epub 2016, Sep 22. PMID:27710939^[6]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Yant LJ, Ran Q, Rao L, Van Remmen H, Shibatani T, Belter JG, Motta L, Richardson A, Prolla TA. The selenoprotein GPX4 is essential for mouse development and protects from radiation and oxidative damage insults. Free Radic Biol Med. 2003 Feb 15;34(4):496-502. PMID:12566075
↑ Seiler A, Schneider M, Forster H, Roth S, Wirth EK, Culmsee C, Plesnila N, Kremmer E, Radmark O, Wurst W, Bornkamm GW, Schweizer U, Conrad M. Glutathione peroxidase 4 senses and translates oxidative stress into 12/15-lipoxygenase dependent- and AIF-mediated cell death. Cell Metab. 2008 Sep;8(3):237-48. PMID:18762024 doi:http://dx.doi.org/S1550-4131(08)00212-X
↑ Schneider M, Forster H, Boersma A, Seiler A, Wehnes H, Sinowatz F, Neumuller C, Deutsch MJ, Walch A, Hrabe de Angelis M, Wurst W, Ursini F, Roveri A, Maleszewski M, Maiorino M, Conrad M. Mitochondrial glutathione peroxidase 4 disruption causes male infertility. FASEB J. 2009 Sep;23(9):3233-42. doi: 10.1096/fj.09-132795. Epub 2009 May 5. PMID:19417079 doi:http://dx.doi.org/10.1096/fj.09-132795
↑ Ueta T, Inoue T, Furukawa T, Tamaki Y, Nakagawa Y, Imai H, Yanagi Y. Glutathione peroxidase 4 is required for maturation of photoreceptor cells. J Biol Chem. 2012 Mar 2;287(10):7675-82. doi: 10.1074/jbc.M111.335174. Epub 2011 , Dec 29. PMID:22207760 doi:http://dx.doi.org/10.1074/jbc.M111.335174
↑ Matsushita M, Freigang S, Schneider C, Conrad M, Bornkamm GW, Kopf M. T cell lipid peroxidation induces ferroptosis and prevents immunity to infection. J Exp Med. 2015 Apr 6;212(4):555-68. doi: 10.1084/jem.20140857. Epub 2015 Mar 30. PMID:25824823 doi:http://dx.doi.org/10.1084/jem.20140857
↑ Janowski R, Scanu S, Niessing D, Madl T. Crystal and solution structural studies of mouse phospholipid hydroperoxide glutathione peroxidase 4. Acta Crystallogr F Struct Biol Commun. 2016 Oct 1;72(Pt 10):743-749. Epub 2016, Sep 22. PMID:27710939 doi:http://dx.doi.org/10.1107/S2053230X16013686

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5l71"

@@ Line 1: / Line 1: @@
 ==Crystal structure of mouse phospholipid hydroperoxide glutathione peroxidase 4 (GPx4)==
-<StructureSection load='5l71' size='340' side='right' caption='[[5l71]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
+<StructureSection load='5l71' size='340' side='right'caption='[[5l71]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5l71]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L71 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5L71 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5l71]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L71 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5L71 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Gpx4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phospholipid-hydroperoxide_glutathione_peroxidase Phospholipid-hydroperoxide glutathione peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.12 1.11.1.12] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5l71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l71 OCA], [http://pdbe.org/5l71 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5l71 RCSB], [http://www.ebi.ac.uk/pdbsum/5l71 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5l71 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5l71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l71 OCA], [http://pdbe.org/5l71 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5l71 RCSB], [http://www.ebi.ac.uk/pdbsum/5l71 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5l71 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 23: / Line 24: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
+[[Category: Lk3 transgenic mice]]
 [[Category: Phospholipid-hydroperoxide glutathione peroxidase]]
 [[Category: Janowski, R]]

5l71

From Proteopedia

Revision as of 06:59, 5 August 2020

Crystal structure of mouse phospholipid hydroperoxide glutathione peroxidase 4 (GPx4)

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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