1prv
From Proteopedia
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'''PURINE REPRESSOR DNA-BINDING DOMAIN DNA BINDING''' | '''PURINE REPRESSOR DNA-BINDING DOMAIN DNA BINDING''' | ||
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[[Category: Schumacher, M A.]] | [[Category: Schumacher, M A.]] | ||
[[Category: Yamamoto, H.]] | [[Category: Yamamoto, H.]] | ||
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Revision as of 02:24, 3 May 2008
PURINE REPRESSOR DNA-BINDING DOMAIN DNA BINDING
Overview
BACKGROUND: The purine repressor (PurR) regulates genes that encode enzymes for purine biosynthesis. PurR has a two domain structure with an N-terminal DNA-binding domain (DBD) and a C-terminal corepressor-binding domain (CBD). The three dimensional structure of a ternary complex of PurR bound to both corepressor and a specific DNA sequence has recently been determined by X-ray crystallography. RESULTS: We have determined the solution structure of the PurR DBD by NMR. It contains three helices, with the first and second helices forming a helix-turn-helix motif. The tertiary structure of the three helices is very similar to that of the corresponding region in the ternary complex. The structure of the hinge helical region, however, which makes specific base contacts in the minor groove of DNA, is disordered in the DNA-free form. CONCLUSION: The stable formation of PurR hinge helices requires PurR dimerization, which brings the hinge regions proximal to each other. The dimerization of the hinge helices is likely to be controlled by the CBD dimerization interface, but is induced by specific-DNA binding.
About this Structure
1PRV is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structural comparison of the free and DNA-bound forms of the purine repressor DNA-binding domain., Nagadoi A, Morikawa S, Nakamura H, Enari M, Kobayashi K, Yamamoto H, Sampei G, Mizobuchi K, Schumacher MA, Brennan RG, et al., Structure. 1995 Nov 15;3(11):1217-24. PMID:8591032 Page seeded by OCA on Sat May 3 05:24:49 2008
