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5h0s

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'''Unreleased structure'''
 
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The entry 5h0s is ON HOLD until Paper Publication
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==EM Structure of VP1A and VP1B==
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<StructureSection load='5h0s' size='340' side='right' caption='[[5h0s]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5h0s]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H0S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5H0S FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5h0r|5h0r]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5h0s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h0s OCA], [http://pdbe.org/5h0s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h0s RCSB], [http://www.ebi.ac.uk/pdbsum/5h0s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5h0s ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Single-particle cryo-electron microscopy (cryo-EM) allows the high-resolution structural determination of biological assemblies in a near-native environment. However, all high-resolution (better than 3.5A) cryo-EM structures reported to date were obtained by using 300kV transmission electron microscopes (TEMs). We report here the structures of a cypovirus capsid of 750-A diameter at 3.3-A resolution and of RNA-dependent RNA polymerase (RdRp) complexes within the capsid at 3.9-A resolution using a 200-kV TEM. The newly resolved structure revealed conformational changes of two subdomains in the RdRp. These conformational changes, which were involved in RdRp's switch from non-transcribing to transcribing mode, suggest that the RdRp may facilitate the unwinding of genomic double-stranded RNA. The possibility of 3-A resolution structural determinations for biological assemblies of relatively small sizes using cryo-EM at 200kV was discussed.
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Authors:
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Near-Atomic Resolution Structure Determination of a Cypovirus Capsid and Polymerase Complex Using Cryo-EM at 200kV.,Li X, Zhou N, Chen W, Zhu B, Wang X, Xu B, Wang J, Liu H, Cheng L J Mol Biol. 2017 Jan 6;429(1):79-87. doi: 10.1016/j.jmb.2016.11.025. Epub 2016, Dec 1. PMID:27914893<ref>PMID:27914893</ref>
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Description:
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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<div class="pdbe-citations 5h0s" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Chen, W]]
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[[Category: Cheng, L]]
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[[Category: Li, X]]
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[[Category: Liu, H]]
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[[Category: Wang, J]]
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[[Category: Wang, X]]
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[[Category: Xu, B]]
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[[Category: Zhou, N]]
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[[Category: Zhu, B]]
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[[Category: Structural classification]]
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[[Category: Transferase]]

Revision as of 23:29, 25 January 2017

EM Structure of VP1A and VP1B

5h0s, resolution 3.30Å

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