1pxu
From Proteopedia
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[[Image:1pxu.gif|left|200px]] | [[Image:1pxu.gif|left|200px]] | ||
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'''Crystal structure of chicken NtA from a eukaryotic source at 2.2A resolution''' | '''Crystal structure of chicken NtA from a eukaryotic source at 2.2A resolution''' | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Stetefeld, J.]] | [[Category: Stetefeld, J.]] | ||
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| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:37:21 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 02:37, 3 May 2008
Crystal structure of chicken NtA from a eukaryotic source at 2.2A resolution
Overview
Agrin is a key organizer for postsynaptic differentiation at the neuromuscular junction (NMJ). This activity requires the binding of agrin to the synaptic basal lamina via its N-terminal (NtA) domain. It has been suggested that this binding is mediated by conserved amino acids in the gamma 1 chain of laminin. Here, we report the crystal structure of chicken NtA expressed in eukaryotic HEK293 cells. In contrast to the previously published structure [Stetefeld, J., Jenny, M., Schulthess, T., Landwehr, R., Schumacher, B., Frank, S., Ruegg, M.A., Engel, J., Kammerer, R.A., 2001. The laminin-binding domain of agrin is structurally related to N-TIMP-1. Nat. Struct. Biol., 8, 705-709.], which was derived from the NtA domain expressed in E. coli, the new data show that the N-terminal tail region (amino acid residues Asn1-Arg5) is highly structured. Moreover, the disulfide bridge between Cys2 and Cys74 was also present. In addition, we show that the binding of NtA requires the gamma 1 chain of laminin and is not greatly affected by the composition of beta chains. These results confirm a model of the NtA-laminin complex where conserved amino acids in the gamma 1 chain are prerequisite for the binding to agrin and they further emphasize that the source of protein can be critical in structure determination.
About this Structure
1PXU is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
Reference
Structure and laminin-binding specificity of the NtA domain expressed in eukaryotic cells., Mascarenhas JB, Ruegg MA, Sasaki T, Eble JA, Engel J, Stetefeld J, Matrix Biol. 2005 Jan;23(8):507-13. Epub 2004 Dec 30. PMID:15694127 Page seeded by OCA on Sat May 3 05:37:21 2008
