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1py0

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{{Structure
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The line below this paragraph, containing "STRUCTURE_1py0", creates the "Structure Box" on the page.
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=Y1:YTTRIUM+ION'>Y1</scene>, <scene name='pdbligand=YMA:7,10,13-TRI(CARBOXYMETHYL)-5,15-DIOXO-4,7,10,13,16-PENTAAZA-1,19-DITHIANONADECANE'>YMA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1py0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1py0 OCA], [http://www.ebi.ac.uk/pdbsum/1py0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1py0 RCSB]</span>
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'''Crystal structure of E51C/E54C Psaz from A.faecalis with CLaNP probe'''
'''Crystal structure of E51C/E54C Psaz from A.faecalis with CLaNP probe'''
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[[Category: Tocheva, E.]]
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[[Category: Ubbink, M.]]
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[[Category: cupredoxin]]
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[[Category: Cupredoxin]]
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[[Category: nmr probe]]
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[[Category: Nmr probe]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:37:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:06:05 2008''
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Revision as of 02:37, 3 May 2008

Image:1py0.gif

Template:STRUCTURE 1py0

Crystal structure of E51C/E54C Psaz from A.faecalis with CLaNP probe


Overview

A lanthanide complex, named CLaNP (caged lanthanide NMR probe) has been developed for the characterisation of proteins by paramagnetic NMR spectroscopy. The probe consists of a lanthanide chelated by a derivative of DTPA (diethylenetriaminepentaacetic acid) with two thiol reactive functional groups. The CLaNP molecule is attached to a protein by two engineered, surface-exposed, Cys residues in a bidentate manner. This drastically limits the dynamics of the metal relative to the protein and enables measurements of pseudocontact shifts. NMR spectroscopy experiments on a diamagnetic control and the crystal structure of the probe-protein complex demonstrate that the protein structure is not affected by probe attachment. The probe is able to induce pseudocontact shifts to at least 40 A from the metal and causes residual dipolar couplings due to alignment at a high magnetic field. The molecule exists in several isomeric forms with different paramagnetic tensors; this provides a fast way to obtain long-range distance restraints.

About this Structure

1PY0 is a Single protein structure of sequence from Alcaligenes faecalis. Full crystallographic information is available from OCA.

Reference

A caged lanthanide complex as a paramagnetic shift agent for protein NMR., Prudencio M, Rohovec J, Peters JA, Tocheva E, Boulanger MJ, Murphy ME, Hupkes HJ, Kosters W, Impagliazzo A, Ubbink M, Chemistry. 2004 Jul 5;10(13):3252-60. PMID:15224334 Page seeded by OCA on Sat May 3 05:37:46 2008

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