1pyv
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1pyv.gif|left|200px]] | [[Image:1pyv.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1pyv", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | | | + | --> |
| - | | | + | {{STRUCTURE_1pyv| PDB=1pyv | SCENE= }} |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''NMR solution structure of the mitochondrial F1b presequence peptide from Nicotiana plumbaginifolia''' | '''NMR solution structure of the mitochondrial F1b presequence peptide from Nicotiana plumbaginifolia''' | ||
| Line 23: | Line 20: | ||
==Reference== | ==Reference== | ||
NMR solution structure of the mitochondrial F1beta presequence from Nicotiana plumbaginifolia., Moberg P, Nilsson S, Stahl A, Eriksson AC, Glaser E, Maler L, J Mol Biol. 2004 Mar 5;336(5):1129-40. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15037074 15037074] | NMR solution structure of the mitochondrial F1beta presequence from Nicotiana plumbaginifolia., Moberg P, Nilsson S, Stahl A, Eriksson AC, Glaser E, Maler L, J Mol Biol. 2004 Mar 5;336(5):1129-40. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15037074 15037074] | ||
| - | [[Category: H(+)-transporting two-sector ATPase]] | ||
[[Category: Nicotiana plumbaginifolia]] | [[Category: Nicotiana plumbaginifolia]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 32: | Line 28: | ||
[[Category: Nilsson, S.]] | [[Category: Nilsson, S.]] | ||
[[Category: Stahl, A.]] | [[Category: Stahl, A.]] | ||
| - | [[Category: | + | [[Category: Hydrolase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:39:25 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 02:39, 3 May 2008
NMR solution structure of the mitochondrial F1b presequence peptide from Nicotiana plumbaginifolia
Overview
We have isolated, characterized and determined the three-dimensional NMR solution structure of the presequence of ATPsynthase F1beta subunit from Nicotiana plumbaginifolia. A general method for purification of presequences is presented. The method is based on overexpression of a mutant precursor containing a methionine residue introduced at the processing site, followed by CNBr-cleavage and purification of the presequence on a cation-exchange column. The F1beta presequence, 53 amino acid residues long, retained its native properties as evidenced by inhibition of in vitro mitochondrial import and processing at micromolar concentrations. CD spectroscopy revealed that the F1beta presequence formed an alpha-helical structure in membrane mimetic environments such as SDS and DPC micelles (approximately 50% alpha-helix), and in acidic phospholipid bicelles (approximately 60% alpha-helix). The NMR solution structure of the F1beta presequence in SDS micelles was determined on the basis of 518 distance and 21 torsion angle constraints. The structure was found to contain two helices, an N-terminal amphipathic alpha-helix (residues 4-15) and a C-terminal alpha-helix (residues 43-53), separated by a largely unstructured 27 residue long internal domain. The N-terminal amphipathic alpha-helix forms the putative Tom20 receptor binding site, whereas the C-terminal alpha-helix is located upstream of the mitochondrial processing peptidase cleavage site.
About this Structure
1PYV is a Single protein structure of sequence from Nicotiana plumbaginifolia. Full crystallographic information is available from OCA.
Reference
NMR solution structure of the mitochondrial F1beta presequence from Nicotiana plumbaginifolia., Moberg P, Nilsson S, Stahl A, Eriksson AC, Glaser E, Maler L, J Mol Biol. 2004 Mar 5;336(5):1129-40. PMID:15037074 Page seeded by OCA on Sat May 3 05:39:25 2008
