Ubiquitin activating enzyme
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
<StructureSection load='1y8x' size='350' side='right' caption='Structure of human ubiquitin activating enzyme Uba3 subunit (green) complex with E2 Ubc12 (grey) (PDB entry [[1y8x]])' scene=''> | <StructureSection load='1y8x' size='350' side='right' caption='Structure of human ubiquitin activating enzyme Uba3 subunit (green) complex with E2 Ubc12 (grey) (PDB entry [[1y8x]])' scene=''> | ||
| - | '''Ubiquitin activating enzyme''' (Uba) or '''E1 enzyme''' catalyzes the first step in the ubiquitination of a protein tagged to be degraded by the proteasome. Ubiquitin activating enzyme (E1) and ATP produce a C-terminal acyl adenylated ubiquitin molecule which binds to ubiquitin conjugating enzyme (E2) (Ubc) cysteine<ref>PMID:7673335</ref>. The Ubc then binds to ubiquitin ligase (E3) via a conserved binding region. E3 catalyzes the transfer of ubiquitin from the Ubc-ubiquitin complex to a lysine residue of the target protein. '''Uba3''' is the catalytic subunit of NEDD8 activating enzyme and APPB1 is its regulatory subunit. Similar to ubiquitin and SUMO, NEDD8 binds to proteins after processing of its C-terminal.<br /> | + | '''Ubiquitin activating enzyme''' (Uba) or '''E1 enzyme''' catalyzes the first step in the ubiquitination of a protein tagged to be degraded by the proteasome. Ubiquitin activating enzyme (E1) and ATP produce a C-terminal acyl adenylated ubiquitin molecule which binds to ubiquitin conjugating enzyme (E2) (Ubc) cysteine<ref>PMID:7673335</ref>. The Ubc then binds to ubiquitin ligase (E3) via a conserved binding region. E3 catalyzes the transfer of ubiquitin from the Ubc-ubiquitin complex to a lysine residue of the target protein. '''Uba3''' is the catalytic subunit of NEDD8 activating enzyme and APPB1 is its regulatory subunit. Similar to ubiquitin and SUMO, NEDD8 binds to proteins after processing of its C-terminal. '''SUMO-activating enzyme subunit 2''' (SAE2) is involved in sumoylation of proteins.<br /> |
For more details on Uba1 see [[Uba1]]. | For more details on Uba1 see [[Uba1]]. | ||
</StructureSection> | </StructureSection> | ||
| Line 41: | Line 41: | ||
**[[3h8v]] – hUba + ATP <BR /> | **[[3h8v]] – hUba + ATP <BR /> | ||
| - | *SUMO-activating enzyme | + | *SUMO-activating enzyme subunit 2 |
| - | **[[3kyc]] – | + | **[[3kyc]] – hSAE2 subunits 1,2 + SUMO <BR /> |
| - | **[[3kyd]] – | + | **[[3kyd]] – hSAE2 subunits 1,2 + SUMO + AMP <BR /> |
| + | **[[5fq2]], [[4w5v]] – hSAE2 ubiquitin domain + Ubc9 <BR /> | ||
}} | }} | ||
==References== | ==References== | ||
<references /> | <references /> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Revision as of 10:59, 30 November 2016
| |||||||||||
3D Structures of ubiquitin activating enzyme
Updated on 30-November-2016
